急性胰腺炎大鼠脾脏核因子κB表达的意义  

The significance of activation of splenic nuclear factor κB in rats with acute pancreatitis

作　　者：陈占峰[1] 陈启龙[1] 

机构地区：[1]新疆医科大学第一附属医院普通外科,新疆乌鲁木齐830054

出　　处：《中国普通外科杂志》2008年第3期233-236,共4页China Journal of General Surgery

摘　　要：目的探讨脾脏在急性胰腺炎(AP)发病中作用。方法实验大鼠随机分成4组:假手术组,脾切除组,AP组,脾切除+AP组。制模后3,6,12h分批处死动物,取材待检。光镜下观察胰腺组织病理改变并进行病理学评分。采用免疫组织化学方法检测大鼠脾脏NF-κB(nuclear factor-κB)p65活化表达水平。结果脾切除+AP组6,12h胰腺病理学评分均低于AP组(6h:7.83±0.753 vs 9.67±1.211;12h:9.67±0.816 vs 13±0.894)(P<0.01);AP大鼠模型中脾脏NF-κBp65活化阳性表达3h即明显(46.967±1.148),6h最强(56.333±1.588),12h表达减弱(36.900±0.756),与假手术组(3.400±0.800)差异均有显著性。结论脾脏可以明显促进炎症介质的产生和释放,在AP发病中起重要作用。Objective To investigate the role of the spleen in the pathogenesis of acute pancreatitis （ AP ）. Methods The studied rats were divided randomly into four groups：Sham operation group, splenectomy group, AP group and AP with splenectomy group. The rats were sacrificed at 3h, 6h and 12h after operation, and spleen and pancreas were removed for examination. Light microscopy was used to examine pancreatic tissues for pathologic change and to assess pathology score. Immunohistochemical method was used to observe the activity of splenic NF-κB（ p65 ） in the rats with AP. Results The pancreas pathology score in the AP with splenectomy group was significantly lower than that in AP group at 6h （7.83 ± 0. 753vs 9.67± 1.211） and 12 h （9.67 ±0.816 vsl3 ±0.894） after operation （P 〈0.01）. The positive expression of splenic NF-KB （ p65 ） began to be intensified at 3 h （ 46. 967 ± 1. 148 ） , peaked at 6h （56.333 21.588） ,attenuated at 12h（36. 900 ± 0. 756） in the rats with AP, and all showed significant differences compared with that in sham operation group （ 3. 400 ± 0. 800 ）. Conclusions The spleen can significantly promote the production and release of inflammatory mediators in AP, which plays an important role in the pathogenesis of AP.

关 键 词：胰腺炎/病理学 脾脏 炎症介质 核因子ΚB 

分 类 号：R657.51[医药卫生—外科学]