不同配比羟基磷灰石/胶原海绵复合物作为组织工程支架材料的比较  

A comparison of 2 kinds of hydroxyapatite/collagen composites as tissue engineering scaffold

作　　者：龚志云[1] 徐志飞[1] 秦雄[1] 赵学维[1] 

机构地区：[1]第二军医大学长征医院胸心外科,上海200003

出　　处：《第二军医大学学报》2008年第3期236-240,共5页Academic Journal of Second Military Medical University

基　　金：国家自然科学基金(30571847)~~

摘　　要：目的：选用不同配比羟基磷灰石和胶原海绵制备成复合材料,研究其结构和生物学特性,为构建组织工程人工胸壁支架材料提供实验依据。方法：按质量比1：1和1：2制备不同配比羟基磷灰石/胶原海绵复合材料,纯胶原海绵作为对照,进行扫描电镜检查,观察材料结构特性;进一步进行体内植入试验,通过大体标本检查及组织学检查观察植入材料生物相容性和降解性能。结果：纯胶原海绵呈三维网状结构,胶原蛋白呈细纤维丝状,结构蓬松,强度差;植入体内2周内多孔海绵结构破坏,4周完全降解吸收。1：2配比羟基磷灰石/胶原海绵复合材料羟基磷灰石粉末与胶原蛋白完全混合均匀,孔径大小100～400μm;植入体内后生物相容性良好,4周时仍保持多孔结构,8周降解吸收。1：1配比复合材料羟基磷灰石粒子团聚成块,植入体内可见明显钙磷颗粒聚集,组织反应相对较大。结论：胶原海绵复合一定比例羟基磷灰石后,结构改善,孔径大小适中,在体内保持结构时间及降解时间延长,生物相容性良好,具备开发应用价值,可作为支架材料应用于组织工程人工胸壁的研究。Objective： To prepare 2 composites using different proportions of hydroxyapatite and collagen and to assess their structural and biological properties, so as to pave a way for preparing tissue engineering chest wall scaffold. Methods： Two kinds of hydroxyapatite/collagen composites were prepared according to the weight ratios of 1 ： 1 and 1 ： 2 ; collagen sponge served as control. Then the structures of the 2 composites and the collagen sponge were observed under SEM. In vivo study was conducted to assess the biocompatibility and biodegradation of the composites by gross inspection and histological examination. Results： The collagen sponge had a 3-D network structure with fluey collagen fibers and poor mechanical strength, and its structure was damaged within 2 weeks after implantation and was completely absorbed 4 weeks later. The hydroxyapatite and collagen were well mixed in the composite with a hydroxyapatite to collagen ratio of 1 ： 2： the composite had homogeneous 3-D porous structure （size of the pore being 100-400 m） and showed good biocompatibility： maintained its porous structure 4 weeks after implantation and was absorbed within 8 weeks. In composite with hydroxyapatite to collagen ratio of 1 ： 1, the hydroxyapatite particles were separated from collagen fiber and conglomerated into masses, and the composite resulted in severe tissue reaction after implantation. Conclusion： When mixed with a reasonable proportion of hydroxyapatite, the collagen sponge has improved structure, biodegradable performance, and biocompatibility; the composite may be a novel scaffold for tissue engineering chest wall reconstruction.

关 键 词：羟基磷灰石类 胶原 生物相容性材料 生物降解 

分 类 号：R655.1[医药卫生—外科学]