盐酸戊乙奎醚对油酸诱导大鼠急性肺损伤作用的影响  

Effect of penehyclidine hydrochloride on oleic acid-induced acute lung injury in rats:a comparison between inhalation and intravenous therapy

作　　者：孔祥[1] 范慧敏[1] 赵曙光[1] 周谦君[1] 卢伟[1] 刘刚[1] 郭建华[1] 刘中民[1] 

机构地区：[1]同济大学附属东方医院中德心脏研究所,上海200120

出　　处：《第二军医大学学报》2008年第3期286-289,共4页Academic Journal of Second Military Medical University

基　　金：上海市科委科研计划资助项目(055407030)~~

摘　　要：目的:观察雾化吸入和静脉注射盐酸戊乙奎醚对油酸诱导大鼠急性肺损伤(acute lung injury,ALI)的影响,并对雾化吸入和静脉用药途径的效果进行比较。方法:采用静脉注射油酸复制ALI大鼠模型。将40只SD大鼠随机分为4组:空白对照组(Ⅰ组)、ALI组Ⅱ组)、盐酸戊乙奎醚雾化吸入治疗组(Ⅲ组)和盐酸戊乙奎醚静脉注射治疗组(Ⅳ组)。实验过程中每隔60min记录大鼠血压(BP)、肺动脉压(PAP),气道压力(airway pressure,Paw)并且检验动脉以及混合静脉血气。注射油酸240min后检测肺湿干比(W/D),测定血清肿瘤坏死因子-α(tumor necrosis factoralpha,TNF-α)水平。结果:Ⅲ、Ⅳ组较之Ⅱ组能够缓解由于注射油酸引起的PAP、Paw升高和PaO2/FiO2下降,Ⅲ组缓解更为明显(P<0.05)。Ⅲ、Ⅳ组W/D,血清TNF-α均比Ⅱ组降低,Ⅲ组降低更为明显(P<0.05)。结论:盐酸戊乙奎醚能够缓解油酸引起的ALI多项指标,相同剂量条件下雾化吸入较之静脉注射途径对ALI有更好的治疗作用。Objective：To compare the therapeutic effects of penehyclidine hydrochloride on rat with oleic acid （OA）-induced acute lung injury （ALI） when given via inhalation and intravenous injection. Methods： Forty male Sprague-Dawley rats were randomly divided into 4 equal groups： normal control group（group D, ALI group（groupⅡ）, penehyclidine hydrochloride inhalation group （group Ⅲ ）, and intravenous penehyclidine hydrochloride group（group Ⅳ）. The blood pressure （BP）, pulmonary artery pressure （PAP）, and airway pressure （Paw） were determined every 60 rain during the experiment and the artery blood gas analysis and mixed venous blood gas analysis were performed. The wet to dry lung weight ratio （W/D） and the serum concentration of tumor necrosis factor alpha （TNF-a） were determined 240 rain after the experiment in each group. Results： The OA-induced increase of PAP,Paw and decrease of PaO2/FiO2 were improved in the group Ⅲ and group Ⅳ compared with those in the group Ⅱ（P0.05 for group Ⅲ）. The W/D and serum TNF-a concentration were lower in the group Ⅲ and group IV compared with those in the group Ⅱ （P〈0.05 for group Ⅲ）. Conclusion： Pene- hyclidine hydrochloride can relieve the symptoms of OA-induced ALI. Inhalation approach is more effective than intravenous injection when the same dose of penehyclidine hydrochloride is given.

关 键 词：急性肺损伤 盐酸戊乙奎醚 动物模型 吸入投药 静脉内注射 

分 类 号：R563[医药卫生—呼吸系统]