一株海洋芽孢杆菌的鉴定及抗菌活性研究  

Identification and antibacterial/antifungal activity of a strain of Bacillus from East China Sea

作　　者：刘小宇[1] 许强芝[1] 韩文菊[1] 卢小玲[1] 刘军华[1] 焦炳华[1] 

机构地区：[1]第二军医大学基础部生物化学与分子生物学教研室,上海200433

出　　处：《第二军医大学学报》2008年第3期321-323,共3页Academic Journal of Second Military Medical University

基　　金：上海市优秀学科带头人计划(05XD14023);国家"863"计划(2006AA09Z416;2007AA091501)~~

摘　　要：目的:对一株编号为ZJ8112的分离自东海海域海泥样品的细菌进行鉴定和抗菌活性研究。方法:采用传统的分类学方法观察菌株ZJ8112的形态、耐盐度,结合16S rDNA序列分析在GenBank上进行比对以确立其进化地位。利用稻瘟霉菌(Pyricularia oryzae)P-2b、枯草芽孢杆菌(Bacillus subtilis)、大肠埃希菌(Escherichia coli)、啤酒酵母(Saccharomy cescerevisi-ae)、白念珠菌(Candida albicans)和黄瓜黑星病菌(Cladosporiumcu cumerinum)作为抗菌活性指示菌,检验ZJ8112的抗菌活性。结果:枯草芽孢杆菌ZJ8112革兰染色阳性,芽孢中生。其最佳生长盐度为10%,属于中等嗜盐菌。通过16S rDNA序列分析发现其与Bacillus subtilis F121112在进化位置上最为接近。该菌株的16SrDNA序列已经提交GenBank,接受号为EU100745。综合ZJ8112菌株的培养特性和形态特征,可认为ZJ8112是一株海洋芽孢杆菌。抗菌活性研究的结果表明其具有很强的抗稻瘟霉菌和抗大肠埃希菌活性。结论:ZJ8112为中等嗜盐的海洋细菌,其最适盐度为10%。生物学活性研究的结果表明,其发酵液具有很强的抗稻瘟霉活性和抗大肠埃希菌活性,具有潜在的抗生素研究的价值。Objective：To identify a strain of bacterium （numbered ZJ8112） isolated from the sediments of the East China Sea and study its antibacterial activity. Methods： The ZJ8l12 strain was subjected to phenotypical study, salt-aggregation test and 16S rDNA sequence analysis; the sequence analysis result was searched in the GenBank to determine its characteristics. The antibacterial activity of strain ZJ8112 was tested using the following as the indicator bacteria： Pyricularia oryzae, Bacillus subtills ,Escherichia coli ,Saccharomyces cerevisiae ,Candida albicans and Cladosporium cucumerinum. Results： We found that the ZJ8l12 was a G＋ bacterium and had central spore. Salt-tolerant study showed that the optimum salt concentration was 10% and it belonged to moderate halophile. Analysis of the 16S rDNA gene showed high similarity （99%） to the Bacillus subtilis F121112. The 16S rDNA sequence of strain ZJ8112 had been submitted to GenBank （accession number： EU100745）. The cultural and phenotypical characteristics demonstrated that ZJ8l12 is a strain of Bacillus. Bioactivity study showed that ZJ8l12 had potent inhibitory activity on Escherichia coli and Pyricularia oryzae. Conclusion： ZJ8112 is a halophytic bacterium with an opti- mum salt concentration of 10%. ZJ8l12 can strongly inhibit the Escherichia coli and Pyricularia oryzae, showing a potential role for antibiotics development.

关 键 词：芽孢杆菌属 16S RDNA序列分析 嗜盐性 抗菌活性 

分 类 号：R282.77[医药卫生—中药学]