肠易激综合征模型大鼠5-羟色胺7受体的表达及作用  

The expression and effect of 5-hydroxytryptamine 7 receptor in brain and intestine of rats with irritable bowel syndrome

作　　者：邹百仓[1] 董蕾[1] 王深皓[1] 曹铭波[1] 王燕[1] 

机构地区：[1]西安交通大学第二医院消化科,710004

出　　处：《中华消化杂志》2008年第3期153-156,共4页Chinese Journal of Digestion

基　　金：国家自然科学基金资助项目（30170414）

摘　　要：目的观察5-羟色胺7受体在肠易激综合征（IBS）不同亚型模型大鼠大脑和消化道组织中表达和分布的差异，探讨其在IBS发病中的作用。方法45只成年SD大鼠分均为对照组、腹泻型IBS（IBS-D）组和便秘型IBS（IBS-C）组。乙酸加束缚应激法制备IBS-D模型，冰水灌胃法制备IBS-C模型。免疫组化法和实时定量PCR法检测各组大鼠大脑、空肠、回肠、近端结肠、远端结肠中5-HT7受体的分布及表达差异。放射免疫法测定以上各组织中环磷酸腺苷（cAMP）含量。结果免疫组化结果显示，IBS-C组和IBS-D组海马及下丘脑、IBS-C组回肠、近端结肠、远端结肠5-HT7受体表达强于对照组（P〈0．05）。实时定量PCR结果显示，IBS-C和IBS-D组海马和下丘脑、IBS-C组回肠、近端结肠及远端结肠5-HT7受体mRNA明显高于对照组（P〈0．05）。IBS-C和IBS-D组海马和下丘脑、IBS-C组近端结肠和远端结肠cAMP含量显著高于对照组（P〈0．05）。结论两组大鼠脑组织及IBS-C大鼠结肠5-HT7受体表达及cAMP水平明显增高，可能与IBS-C胃肠动力障碍和内脏感觉异常有关。Objective To investigate the expression and distribution of 5-hydroxytryptamine 7 （5-HT7） receptor in brain and intestine of rats with different subtype of irritable bowel syndrome（IBS）. Methods Forty-five SD rats were divided into healthy contral, diarrhea-predominant（IBS-D） and constipation-predominant（IBS-C） IBS groups. IBS-D and IBS-C models were established by either colonic instillation of acetic acid and restraint stress or stomach irrigation with cool water（0-4 ℃）. The expression and distribution of 5-HT7 receptor at brain and intestine were determined by immunohistochemistry and real-time PCR. The tissue concentration of cyclic AMP（cAMP） was examined by radioimmunoassay. Results Immunohistochemistry study demonstrated that the staining of 5-HT7 receptor at hippocampus and hypothalamus were strong in IBS-C and IBS-D groups compared to control group（P 〈 0.01）. The staining of 5-HT7 receptor at ileum, proximal colon and distal colon were higher in IBS-C group than those in control group（P 〈 0.05）. Real-time PCR revealed that the expression of 5-HT7 receptor at hippocampus and hypothalamus were higher in IBS-C and IBS-D groups than those in control group（P 〈 0.05）, and the expression at ileum and colon were remarkably higher in IBS-C group compared to control group（P 〈 0.05）. The concentration of cAMP at hippocampus and hypothalamus were higher in IBS-C and IBS-D groups than those in control group（P 〈 0.01, P 〈 0.05）. The cAMP level at proximal and distal colon in IBS-C group was higher than those in control group（P 〈 0. 05）. Conclusion The upregulation of 5-HT7 receptor in brain and intestine may be related with the dyskinesis and visceral pares- thesia of IBS-C.

关 键 词：肠易激综合征 受体 5-羟色胺 环磷酸腺苷 

分 类 号：R686[医药卫生—骨科学]