Kangai-1抑制胰腺癌细胞转移与细胞粘附分子-1和金属蛋白酶-9关系的研究  

The inhibitory effect of Kangai-1 gene on metastasis of pancreatic cancer and its association with intercellular adhesion molecule-1 and matrix metalloproteinases-9

作　　者：郭晓钟[1] 田宏[1] 徐建华[1] 弭延斌[1] 崔忠敏[1] 李宏宇[1] 张巍巍[1] 赵佳军[1] 穆振斌[1] 

机构地区：[1]沈阳军区总医院消化科,沈阳110016

出　　处：《中华消化杂志》2008年第3期175-178,共4页Chinese Journal of Digestion

基　　金：国家自然科学基金资助项目（30470798）

摘　　要：目的研究肿瘤转移抑制基因Kangai-1（KAI1）对胰腺癌细胞增殖功能及转移潜能的影响。方法建立经腺病毒（Ad）成功构建的重组载体（Ad—KAI1），并转染胰腺癌PANCI细胞。用血管内皮生长因子（VEGF）和表皮生长因子（EGF）作为诱导剂，按照不同诱导时间点（3、5、7h）将PANCI细胞进行实验分组，比较感染前后细胞形态。采用Transwell方法对PANCI细胞转染Ad—KAI1前、后的迁移作用进行比较。采用免疫细胞化学法检测细胞粘附分子-1（ICAM-1）和金属蛋白酶-9（MMP-9）在PANCI细胞转染Ad—KAI1前、后的表达水平。结果在VEGF和EGF诱导下，转染Ad—KAI1后与转染前相比，PANCI细胞的迁移能力明显下降（P〈0．05）。免疫细胞化学研究提示，PANCI细胞中ICAM-1、MMP-9均呈阳性表达，转染后均呈阴性表达。结论KAI1基因抑制胰腺癌转移可能是通过抑制ICAM-1和MMP-9的表达。Objective To investigate the underlying mechnisms of Kangai-l（KAI1） gene, a tumor metastasis suppressor gene, on metastasis and prolification of pancreatic cancer cells. Methods The plasmin containing Ad-KAI1 was established and transfected into pancreatic cancer cell line PCNA I. The PCNA I cells were then divided into different groups according to the times induced by vascular endothelial growth factor（VEGF） and epidermal growth factor（EGF）. The morphology and migrational ability of PANC I cells were compared before and after transfection by microscopy and transwell method, respectively. The expressions of intercellular adhesion molecule-1 （ICAM-1） and matrix metalloproteinases-9（MMP-9） in PANC I cells were examined by immunocytochemistry before and after transfection. Results The migrational ability of PCNA I cells tr;ansfected with Ad-KAI1 was siginificantly decreased compared with untransfected PCNA I cells（P 〈 0.05）. Immunocytochemistry study revealed that the expressions of ICAM-1 and MMP-9 were both positive in untransfected PCNA I cells, but were both negative in transfected PCNA I cells. Conclusion The inhibitory mechanism of KAI1 gene on metastasis of pancreatic cancer is associated with down-regulation of ICAM-1 and MMP-9 expressions.

关 键 词：胰腺肿瘤 Kangai-1蛋白 细胞粘附分子-1 金属蛋白酶-9 

分 类 号：R686[医药卫生—骨科学]