Cloning and characterization of a heat shock protein 70 gene, MsHSP70-1, in Medicago sativa  

作　　者：Zhishui He Rong Xie Yanzhang Wang Huasong Zou Jiabi Zhu Guanqiao Yu 

机构地区：[1]National Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China [2]Graduate School of the Chinese Academy of Sciences, Beijing 100049, China

出　　处：《Acta Biochimica et Biophysica Sinica》2008年第3期209-216,共8页生物化学与生物物理学报（英文版）

摘　　要：Some members of the heat shock protein 70 （HSP70） family have important functions in organism development. Here, we identified an alfalfa （Medicago sativa L. cv. Algonquin） HSP70 gene, MsHSP70-1, using cDNA array and reverse transcription-polymerase chain reaction. This gene contains a 1947 bp open reading frame encoding a protein of 649 amino acids. This protein contains all conserved domains, motifs, and characteristic sequences of plant HSP70s. The expression of MsHSP70-1 is enhanced in nodule compared with root, stem, leaf, and flower, and throughout the process of nodule development. Northern hybridization analysis indicated that the expression of MsHSP70-1 in nodule requires the active bacA gene of rhizobia. These results suggested that MsHSP70-1 might play an important role in affaffa nodule development.Some members of the heat shock protein 70 （HSP70） family have important functions in organism development. Here, we identified an alfalfa （Medicago sativa L. cv. Algonquin） HSP70 gene, MsHSP70-1, using cDNA array and reverse transcription-polymerase chain reaction. This gene contains a 1947 bp open reading frame encoding a protein of 649 amino acids. This protein contains all conserved domains, motifs, and characteristic sequences of plant HSP70s. The expression of MsHSP70-1 is enhanced in nodule compared with root, stem, leaf, and flower, and throughout the process of nodule development. Northern hybridization analysis indicated that the expression of MsHSP70-1 in nodule requires the active bacA gene of rhizobia. These results suggested that MsHSP70-1 might play an important role in affaffa nodule development.

关 键 词：heat shock protein 70 cDNA array symbioticnitrogen fixation nodule development 

分 类 号：Q785[生物学—分子生物学]