电压敏感染料di-4-ANEPPS在灌注液中的光谱特性  

Spectral Characteristics of Voltage Sensitive Dye di-4-ANEPPS in Perfusates

作　　者：徐正红[1,2] 张镇西[1] 王晶[1] 张虹[1,2] 

机构地区：[1]西安交通大学生命科学与技术学院,生物医学信息工程教育部重点实验室,西安710049 [2]西安交通大学电气工程学院,西安710049

出　　处：《光子学报》2008年第3期552-554,共3页Acta Photonica Sinica

基　　金：国家自然科学基金资助(60378018和60578026)

摘　　要：研究了电压敏感染料di-4-ANEPPS在溶液中的吸收光谱和荧光光谱特性.结果表明,染料的吸收峰在480nm附近,加入钙阻断剂BDM不会影响染料的吸收峰位.染料在去离子水和新鲜台氏液中的荧光发射光谱峰位近似,而在灌注后的台氏液中染料的荧光峰位有蓝移现象.这些研究结果为心脏光学标测系统的设计提供了理论依据.This study investigated the absorption spectrum and the fluorescence spectrum of the voltage sensitive dye （di-4 ANEPPS） in perfusates. The results indicateds that the maximum absorption peak of the dye was nearly at 480 nm. Additionally, the calcium channel blocker BDM doesn＇t affect the peak of absorption spectrum. Meanwhile, the peak of fluorescence emission spectrum of the dye in the distilled water are same as that in the fresh perfusate, but it is moved to the blue side of the spectrum in the perfusate after perfusion. These results are a theoretical foundation for the design of a cardiac optical mapping system.

关 键 词：光谱学 电压敏感染料 吸收光谱 荧光光谱 光学标测 

分 类 号：O433[机械工程—光学工程]