激活素A和卵泡抑素对冻融人胎儿卵巢雌二醇分泌的影响  

Effect of Activin A-follistatin on steroidogenesis in frozen-thawed human fetal ovary in vitro

作　　者：池海虹[1] 周颖[1] 祝怀平[2] 金畅[3] 叶碧绿[1] 

机构地区：[1]温州医学院第一附属医院生殖医学中心,浙江温州325000 [2]绍兴文理学院附属医院妇科,浙江绍兴312000 [3]温州医学院附属眼视光学院,浙江温州325000

出　　处：《温州医学院学报》2008年第2期109-111,共3页Journal of Wenzhou Medical College

基　　金：国家自然科学基金资助项目(30370749);浙江省科技厅科研基金资助项目(2003C33001);温州市科技计划资助项目(Y2004A032)

摘　　要：目的:研究组织培养条件下冻融人胎儿卵巢雌二醇(E2)的分泌及激活素A(Act A)和卵泡抑素(FS)对E2分泌的影响。方法:冻融人胎儿卵巢组织体外培养,分别加入基础培养液(对照组,即MEM组)、基础培养液+100 ng/ml Act A(Act A组)、基础培养液+100 ng/ml FS(FS组)和基础培养液+100 ng/mlAct A+100 ng/ml FS(A+F组),每组4块组织,培养8 d。收集培养2 d、4 d、6 d、8 d各组培养液,测定比较其E2值。结果:培养4 d、6 d、8 d卵巢组织分泌的E2值均高于2 d的E2值(均P<0.05),随培养时间延长,E2分泌量先增加后减少,在培养后6 d可达较高分泌水平;Act A组E2分泌量明显高于对照组、FS组和A+F组(均P<0.01)。结论:冻融人胎儿卵巢在组织培养条件下能分泌E2,Act A能促进体外培养胎儿卵巢E2的分泌,其作用可能受到FS的调节。Objective：To investigate the effect of Activin A-follistatin on steroidogenesis of frozen-thawed human fetal ovary in vitro. Methods： The frozen-thawed fetal ovarian tissue was cultured individually in minimal essential medium （MEM） for 8 days. Under the experimental conditions, the medium was supplemented with either Activin-A （Act A group） or follistatin （FS group） or the combinations of Activin-A and follistatin （A＋F group）. The concentration of E2 in cultured medium was detected with immunluminescence method. Results： The levels of E2 at 4th day, 6th day and 8th day were higher than that at 2nd day （P〈0.05）, The secretion of E2 increased at first, then decreased with the highest level at 6th day. The level of E2 of Act A group was significantly greater than that of the control group, FS group and A＋F group （P〈0.01）. Conclusion： Frozen-thawed human fetal ovary can excrete estrodiol under the condition of tissue culture. Act A can increase the excretion of estrodiol in cultivated ovarian tissue, and the effection of Act A can be suppressed by FS.

关 键 词：激活素A 卵泡抑素 胎儿 卵巢 雌二醇 

分 类 号：R715.5[医药卫生—妇产科学]