中药苦参碱对体外培养人宫颈癌Hela细胞的抑制作用  

Inhibitory effect of matrine on human cervical carcinoma Hela cells in vitro

作　　者：金畅[1] 滕小玲[2] 雷亚宁[1] 周颖[1] 郑膺[1] 张婵[3] 

机构地区：[1]温州医学院组织胚胎学教研室,浙江温州3250035 [2]温州市第三人民医院妇产科,浙江温州325000 [3]温州医学院生物学实验教学中心,浙江温州325035

出　　处：《温州医学院学报》2008年第2期134-137,共4页Journal of Wenzhou Medical College

基　　金：温州市科技局科研基金资助项目(Y20060103);浙江省教育厅科研基金资助项目(20061829)

摘　　要：目的:探讨中药苦参碱对体外培养的人宫颈癌细胞株Hela细胞的抑制作用。方法:选用人宫颈癌Hela细胞进行体外培养,以0.125～2.0 mg/ml的苦参碱分别处理Hela细胞24～72 h后,MTT法检测细胞增殖,流式细胞仪(FCM)检测细胞凋亡率,透射电镜进行形态学观察,RT-PCR半定量检测凋亡相关基因Bcl-2和Bax的mRNA表达水平。结果:苦参碱对Hela细胞有抑制作用,呈剂量和时间依赖性,其24 h半数抑制浓度(IC 50)为1.074 mg/ml;流式细胞仪检测到细胞在苦参碱作用后,细胞凋亡率增加;透射电镜观察发现部分细胞发生凋亡早期改变;RT-PCR结果显示凋亡相关基因Bcl-2/Bax比值随药物剂量增加而逐渐减少。结论:苦参碱对人宫颈癌Hela细胞具有明显抑制作用,诱导细胞凋亡可能是其机制之一。Objective：To explore the inhibitory effect of matrine on human cervical carcinoma Hela cells in vitro. Methods： The Hela cells were cultured in vitro and treated with different concentrations （0.125- 2.0mg/ml） of matrine solution for 24- 72 hours. MTT assay was performed to evaluate the cytotoxin effect of matrine on Hela cells. FCM was used to detect the apoptosis of Hela cells. Transmission Electron Microscope analyses were carried out to examine the cell morphology. Semiquantitative RT-PCR was assessed to detect the mRNA levels of Bcl-2 and Bax gene expressions. Results： Different concentrations of matrines were cytotoxic to Hela cell in time-dependent and dose-dependent manner. The FCM analysis showed that the apoptosis rate increased after Hela cells were effected by matrine. Partial cell spresented characteristic morphological changes under the transmission electron microscope. The results of RT-PCR showed that the rate of Bcl-2/Bax decreased when the matrine concentration increased. Conclusion： Matrine can inhibit the proliferation of human cervical carcinoma Hela cells in vitro. Inducing apoptosis may be one of the mechanisms.

关 键 词：苦参碱 宫颈癌 凋亡 

分 类 号：R28[医药卫生—中药学] R711.74[医药卫生—中医学]