应用RT-Realtime PCR检测南芥菜花叶病毒  

Method for detecting Arabis mosaic virus by RT-Realtime PCR

作　　者：邓丛良[1] 吕玉峰[1] 梁新苗[1] 汪万春[1] 高文娜[1] 

机构地区：[1]北京出入境检验检疫局,100026

出　　处：《植物检疫》2008年第2期80-82,共3页Plant Quarantine

基　　金：国家质检总局课题:北京奥运会入境植物检疫监测体系的建立及关键技术研究(2007IK230)

摘　　要：根据南芥菜花叶病毒不同分离物外壳蛋白(CP)基因的保守序列,设计合成引物和1条TaqMan荧光探针,建立了ArMV的反转录实时荧光PCR(RT-Realtime PCR)检测方法。该方法利用TaqMan荧光探针实时监测目的基因的扩增,实现PCR扩增与探针检测同时进行,实验结果表明该方法具有"灵敏、准确、简便、快速"的特点,适合于口岸对南芥菜花叶病毒的快速检测。A pair of primers and a TaqMan MAG probe based on the conserved nucleotide sequence of coat protein （CP） gene of several Arabis mosaic virus （ArMV） isolates were designed and synthesized. A novel real - time fluorescent RT - PCR was established to detect ArMV. The technology uses a TaqMan probe to monitor in real -time amplification of the target gene. The method provides a rapid, sensitive and specific detection of ArMV and is suitable for quarantine detection.

关 键 词：南芥菜花叶病毒(ArMV) 实时荧光RT-PCR 检测 

分 类 号：S432.4[农业科学—植物病理学] S41[农业科学—农业昆虫与害虫防治]