利用链霉素耐药性突变筛选抗菌活性放线菌  

Studies on the Screening of Bioactive Actinomycetes by Inducing Streptomycin Resistance Mutation

作　　者：沈玲[1] 郭正彦[1] 姬志勤[1] 龙建友[2] 胡兆农[1] 吴文君[1] 

机构地区：[1]西北农林科技大学农药研究所,陕西杨凌712100 [2]广州大学环境科学与工程学院,广州510006

出　　处：《农药学学报》2008年第1期61-67,共7页Chinese Journal of Pesticide Science

基　　金：国家重点基础研究发展规划(“973”计划)项目(2003CB114404)

摘　　要：利用链霉素耐药性突变,从天然无抗菌活性或活性微弱的12株放线菌中筛选活性菌株。在链霉素浓度为25μg/mL时共获得99株耐药菌株。通过抑菌活性测定,发现7株耐药突变菌株的发酵液产生了抑制细菌活性,其中仅耐药突变株67S-14所产生活性物质的遗传性能稳定。室内抑制真菌活性测定结果表明:菌株67S-14发酵液对玉米大斑病菌Exserohilum turcicum、番茄灰霉病菌Botrytis cinerea和棉花枯萎病菌Fusarium oxysporumf.sp.vasinfectum的抑制率较原始菌株No.67提高50%以上。该突变株在菌落形态方面与原始菌株存在较大的差异。Doskochilova溶剂系统纸层析结果表明,该活性物质可能为大环内酯类抗生素。HPLC分析结果表明,该抗生素可能是突变菌株67S-14产生的代谢产物。The activation experiments of twelve natural inactive actinomycetes by inducing streptomycin-resistance mutation were carried out. Ninety-nine mutants were obtained at streptomycin concentration of 25 μg/mL （MIC）. Seven mutants showed bioactivity against tested bacterial were screened out,and one of them--the strain 67S-14 exhibited strong and constant inhibition effect. The results of antifungal activity in vitro showed that the inhibitory rate of fermentation broth of the strain 67S-14 was more than 50% higher than that of the wild strain No. 67 against Exserohilum turcicu, Botrytis cinerea and Fusarium oxysporum f. sp. vasinfectum. The mutant 67S-14 showed obvious differences in the morphological characteristics compared with the wild-type strain No. 67. The results of paper layer chromatography demonstrated that the bioactive substance might be a macrolide antibiotic. HPLC analysis showed that the antibiotic might be a metabolite of mutant 67S-14.

关 键 词：链霉素 耐药性突变 放线菌 活性 大环内酯类抗生素 

分 类 号：S482.28[农业科学—农药学]