Sperm quality improvement after natural anti-oxidant treatment of asthenoteratospermic men with leukocytospermia  

作　　者：Paola Piomboni Laura Gambera Francesca Serafini Giovanna Campanella Giuseppe Morgante Vincenzo De Leo 

机构地区：[1]Department of Surgery, Biology Section,Obstetrics and Reproductive Medicine, Gynecology and Obstetrics Section, University of Siena, Siena 53100, Italy [2]Center for Diagnosis and Treatment of Couple Sterility, Siena Hospital, Siena 53100, Italy [3]Department of Pediatrics, Obstetrics and Reproductive Medicine, Gynecology and Obstetrics Section, University of Siena, Siena 53100, Italy

出　　处：《Asian Journal of Andrology》2008年第2期201-206,共6页亚洲男性学杂志（英文版）

摘　　要：Aim： To study the immune-modulating and anti-oxidant effects of beta-glucan, papaya, lactoferrin, and vitamins C and E on sperm characteristics of patients with asthenoteratozoospermia associated with leucocytosis. Methods： Fifty-one patients referred to our Sterility Center for semen analysis were selected. Sperm parameters were assessed before and after patient＇s treatment with beta-glucan, lactoferrin, papaya, and vitamins C and E. DNA damage was assessed by the acridine orange test and sperm structural characteristics were evaluated by transmission electron microscopy. Results： After 90 days of treatment, an increase in the percentage of morphologically normal sperm （17.0 ± 5.2 vs. 29.8 ± 6.5） and total progressive motility （19.0± 7.8 vs. 34.8 ± 6.8） were detected. Structural sperm characteristics as well as chromatin integrity were also improved after treatment. In terms of leukocyte concentration in seminal fluid, a significant reduction was recorded （2.2 ± 0.9 vs. 0.9± 0.2）. Conclusion： The treatment of an inflammatory process by the synergic action of immune modulators and anti-oxidants could protect sperm during maturation and migration, leading to improved sperm function. （Asian JAndrol 2008 Mar;. 10： 201-206）Aim： To study the immune-modulating and anti-oxidant effects of beta-glucan, papaya, lactoferrin, and vitamins C and E on sperm characteristics of patients with asthenoteratozoospermia associated with leucocytosis. Methods： Fifty-one patients referred to our Sterility Center for semen analysis were selected. Sperm parameters were assessed before and after patient＇s treatment with beta-glucan, lactoferrin, papaya, and vitamins C and E. DNA damage was assessed by the acridine orange test and sperm structural characteristics were evaluated by transmission electron microscopy. Results： After 90 days of treatment, an increase in the percentage of morphologically normal sperm （17.0 ± 5.2 vs. 29.8 ± 6.5） and total progressive motility （19.0± 7.8 vs. 34.8 ± 6.8） were detected. Structural sperm characteristics as well as chromatin integrity were also improved after treatment. In terms of leukocyte concentration in seminal fluid, a significant reduction was recorded （2.2 ± 0.9 vs. 0.9± 0.2）. Conclusion： The treatment of an inflammatory process by the synergic action of immune modulators and anti-oxidants could protect sperm during maturation and migration, leading to improved sperm function. （Asian JAndrol 2008 Mar;. 10： 201-206）

关 键 词：asthenoteratozoospermia LEUKOCYTOSPERMIA ANTI-OXIDANT BETA-GLUCAN PAPAYA LACTOFERRIN vitamin C vitamin E chromatinintegrity transmission electron microscopy 

分 类 号：R69[医药卫生—泌尿科学]