Atomic force microscopic view of the fine topography on the tobacco stigma surface during its response to pollination  

作　　者：WU Hui LI Meng SUN MengXiang 

机构地区：[1]Key Laboratory of MOE for Plant Developmental Biology, College of Life Science, Wuhan University, Wuhan 430072, China [2]Department of Biological Engineering, Henan Institute of Science and Technology, Xinxiang 453003, China

出　　处：《Chinese Science Bulletin》2008年第7期1015-1020,共6页

基　　金：the National Natural Science Foundation of China (Grant No. 90408002);the Key Grant Project of Chinese Ministry of Education (Grant No.307018);Open Project of Key Laboratory of MOE for Plant Developmental Biology

摘　　要：During compatible pollination in tobacco, an extracellular matrix (ECM) is secreted from the stigma surface; however, it is unknown whether the pattern of secretion across the stigma depends on the pollen source. In fact, technical limitations have prevented clear observation of ECM secretion. Here, we report the detailed topographic changes on the stigma surface that accompanies intraspecies and interspecies pollination in tobacco using contact mode atomic force microscopy (AFM). Our results, which show the dynamics and time course of ECM secretion after pollination, indicate that a certain pattern of secretion already exists on the stigma prior to pollination. Intraspecies induced a two-step response, characterized by topographical changes on the stigma surface several hours after pollina-tion, which was distinct from the pattern of ECM secretion induced by interspecies pollination. This difference was confirmed by root-mean-square analysis, which assessed the roughness of the stigma surface. Our findings indicate that compatible pollination not only induces ECM secretion from the stigma, but also results in a specific distribution of the ECM. Thus, this study demonstrates the pow-erful potential of AFM in studying the pollen-stigma interaction.During compatible pollination in tobacco, an extracellular matrix （ECM） is secreted from the stigma surface; however, it is unknown whether the pattern of secretion across the stigma depends on the pollen source. In fact, technical limitations have prevented clear observation of ECM secretion. Here, we report the detailed topographic changes on the stigma surface that accompanies intraspecies and interspecies pollination in tobacco using contact mode atomic force microscopy （AFM）. Our results, which show the dynamics and time course of ECM secretion after pollination, indicate that a certain pattern of secretion already exists on the stigma prior to pollination. Intraspecies induced a two-step response, characterized by topographical changes on the stigma surface several hours after pollination, which was distinct from the pattern of ECM secretion induced by interspecies pollination. This difference was confirmed by root-mean-square analysis, which assessed the roughness of the stigma surface. Our findings indicate that compatible pollination not only induces ECM secretion from the stigma, but also results in a specific distribution of the ECM. Thus, this study demonstrates the powerful potential of AFM in studying the pollen-stigma interaction.

关 键 词：原子动力学 烟草制品 气门 花粉 

分 类 号：TS4[农业科学—烟草工业]