不明原因反复自然流产患者种植窗期子宫内膜胞饮小泡的密度及黏蛋白-1的表达  

Density of pinopodes and expression of mucin-1 in patients with unexplained recurrent miscarriage during implantation window phase

作　　者：徐步芳[1] 孙晓溪[1] 李路[1] 吴煜[1] 高晓红[1] 程利南[1] 

机构地区：[1]上海交通大学医学院国际和平妇幼保健院生殖医学中心,上海200030

出　　处：《上海交通大学学报（医学版）》2008年第3期243-246,共4页Journal of Shanghai Jiao tong University:Medical Science

基　　金：上海市科委基金(034119861)~~

摘　　要：目的分析不明原因反复自然流产(URM)患者种植窗期子宫内膜上皮细胞表面胞饮小泡及种植因子黏蛋白-1(MUC-1)表达,探讨URM与子宫内膜接受性异常的相关性。方法应用扫描电镜观察及免疫组化染色,分析URM患者(n=24)及正常生育妇女(n=22)种植窗期子宫内膜胞饮小泡形态和密度的改变及MUC-1的表达。结果URM患者种植窗期子宫内膜上皮细胞表面胞饮小泡的密度明显低于正常生育妇女(P<0.01);URM患者成熟期胞饮小泡所占比例明显减少;MUC-1在URM患者子宫内膜腺上皮细胞和腔上皮细胞的表达也明显低于正常生育妇女(P<0.05)。结论URM患者种植窗期子宫内膜成熟期胞饮小泡数量减少,胞饮小泡总量下降,MUC-1表达下降,提示URM患者种植窗期子宫内膜接受性下降。Objective To analyse the pinopodes and mucin-l（MUC-1） expression in unexplained recurrent miscarriage （URM） patients during implantation window phase, and investigate the relationship between URM and abnormal endometrial receptivity. Methods Endometrial biopsies were obtained from URM patients （experiment group, n = 24） and normal fertile women （control group, n=22）. Scanning electron microscope and immunohistochemistry were used for analysis of configuration and density of pinopodes and MUC-1 expression during implantation window phase. Results The presence of fully developed pinopodes and density of pinopodes on endometrial surface in the experiment group were much lesser than those in the control group （ P 〈 0.01 ）. Besides, the expression of MUC-1 on the endometrial glandular epithelial cells and luminal epithelial cells was significantly less in the experiment group than that in the control group （ P〈0.05）. Conclusion The number of fully developed pinopodes, the total number of pinopodes and the expression of MUC-1 were decreased in URM patients during implantation window phase, indicating a decreased endometrial receptivity in URM patients during implantation window phase.

关 键 词：子宫内膜 种植窗 反复自然流产 胞饮小泡 黏蛋白-1 

分 类 号：R714.21[医药卫生—妇产科学]