骨保护素基因敲除对小鼠左心室收缩功能的影响  

Effect of OPG knockout on left ventricular systolic function of mouse

作　　者：李西华[1] 颜美珠[2] 许勇[3] 庞晓芬[2] 王铸钢[1] 

机构地区：[1]上海交通大学基础医学院医学遗传学教研室 [2]上海交通大学医学院瑞金医院老年科,上海200025 [3]上海交通大学医学院瑞金医院伤骨科研究所,上海200025

出　　处：《上海交通大学学报（医学版）》2008年第3期285-288,共4页Journal of Shanghai Jiao tong University:Medical Science

基　　金：国家高技术研究发展计划(2001AA216081,2004AA216081);国家自然基金杰出青年科学家基金(39925023);国家教育部基金(00TPJS1111);上海市科技发展基金(99JC14029,99XD14005);上海市教育局E-研究院基金(E03003)~~

摘　　要：目的探讨左心室收缩功能与骨保护素配体(RANKL)和雌激素在骨保护素(OPG)基因敲除小鼠引起骨质疏松之间的关系。方法建立OPG基因敲除小鼠模型,并设对照组;ELISA法检测小鼠血清OPG和RANKL水平,放射免疫法检测血清雌激素水平;利用心脏超声对心功能进行评价。结果与对照组比较,OPG基因敲除组小鼠的心脏质量、心脏质量与体质量的比值、左室心肌细胞的横切面积均明显增大(P<0.01),左心室收缩末期内径和左心室收缩末期容积也明显增大(P<0.05)。OPG基因敲除组小鼠血清RANKL浓度明显高于对照组(P<0.001),两组血清雌激素水平则无显著差异(P>0.05)。结论OPG缺乏导致血清RANKL水平升高,左心室心肌细胞肥大,但不影响血清雌激素水平。Objective To investigate the relationships between serum levels of receptor activator of nuclear factor-kappa B ligand （ RANKL）, estrogen and heart function in osteoprotegerin （OPG） deficience-induced mouse osteoporosis. Methods OPG knockout mouse model and control group were established. Serum OPG and RANKL levels were measured by ELISA. Serum estrogen was detected by radioimmunology, and cardiac ultrasonography was performed to assess the heart function. Results The heart weight, the ratio of heart weight to body weight and transverse area of myocardial cells of left ventricle were significantly increased in OPG knockout mice compared to the control group （ P 〈 0.01 ）. Left ventricular systolic diameter and left ventricular end-systolic volume in OPG knockout mice were significantly higher than those in the control group（P 〈 0.05）. The serum RANKL level in OPG knockout mice was higher than that of the control group （P 〈 0. 001 ）, while there was no significant change in the serum estrogen level（P 〉 0.05）. Conclusion Deficience of OPG induces up-regulation of serum RANKL level and myocardial hypertrophy, but has no effect on serum estrogen level.

关 键 词：骨保护素 骨保护素配体 左心室 小鼠 

分 类 号：Q3[生物学—遗传学]