铅中毒大鼠血清一氧化氮含量与血清酶活性逆向变化的关系  

Relationship between the content of NO and the reversed change of enzyme in serum of rats with lead poisoning.

作　　者：陈森洲[1] 徐雅娟[1] 王险峰[1] 袁桂峰[1] 骆耐香[1] 裴蕾[1] 

机构地区：[1]桂林医学院

出　　处：《华中医学杂志》2007年第6期475-476,共2页Central China Medical Journal

基　　金：广西科技厅自然科学基金资助项目(No0640184)

摘　　要：目的探讨大鼠铅中毒后,其血清NO含量与几种血清酶活性逆向变化的关系。方法Wistar雌性大鼠64只,随机分为对照组、10mg/kg、30mg/kg和60mg/kg铅组。醋酸铅腹腔注射,隔天1次,7次后处死,测血铅含量、血清中NO含量和丙氨酸转氨酶(ALT)、碱性磷酸酶(ALP)、天门冬氨酸转氨酶(AST)、乳酸脱氢酶(LDH)及γ-谷氨酰转移酶(γ-GT)的活性,并作肝组织病理检查;另分别向大鼠肝匀浆中加入不同浓度的NaNO2,测定血清酶活性。结果染铅剂量增加,血清NO和血铅的含量升高,ALT、AST和γ-GT活性呈现上升趋势,而ALP和LDH活性呈现下降趋势;肝细胞未见明显的损害。体外实验表明,NaNO2对ALT、AST及LDH的活性均有抑制作用,而对γ-GT及ALP无抑制作用。结论铅中毒大鼠血清酶活性的逆向变化可能为NO等因素对酶活性存在不同程度的抑制所导致。Objective To study the relationship between NO content and the reversed change of enzyme activities in serurn of rats with lead poisoning. Methods Female Wistar rats were randomly divided into four groups： The control group treated with distilled water only; the experimental groups treated with 10 mg/kg PbAc2, 30 mg/kg PbAc2 and 60 mg/kg PbAc2, respectively, every two days for 7 times. The content of NO and the activity of ALT, ALP and γ-GT in serum were measured. The hepatic cells of the rats were examined pathomorphologically. The activity of sero-enzyme was measured in the liver homogenate after addition of different concentrations of NaNO2. Results With the increase of lead dose, the content of NO and lead were significantly increased, and the activity of ALT, AST and γ-GT in serum significantly increased, while the activity of ALP and LDH significantly increased. The histopathological examination revealed that there was no obvious damage to the liver cells. In vitro, the activity of ALT, AST and LDH was inhibited by NaNO2 concentration to some degrees. Conclusion The reversed change of enzyme activity in serum of rats with lead poisoning might be contributed to the inhibition of enzyme activity to varying degrees by NO.

关 键 词：铅中毒 一氧化氮 血清酶活性 

分 类 号：R595.2[医药卫生—内科学]