T细胞比例对大鼠移植物抗宿主反应的影响  

Study on the influence of T cell ratio between graft and recipient on graft-versus-host disease in rats

作　　者：梁辉[1] 翁霞[1] 邓晓辉[1] 

机构地区：[1]上海交通大学医学院附属新华医院血液科,上海200092

出　　处：《内科理论与实践》2008年第2期123-125,共3页Journal of Internal Medicine Concepts & Practice

基　　金：上海血液学研究所胡应洲基金科研基金项目资助(2005)

摘　　要：目的:研究移植物和受体的T细胞比例与移植物抗宿主反应(GVHD)的关系。方法:受体SD大鼠和供体Wistar大鼠共40对,按移植物与受体的T细胞比例1∶1、2∶1、4∶1和对照组分为4组进行移植,并观察移植后GVHD的发生及其程度。结果:4组大鼠中1∶1和2∶1组的GVHD最轻,与对照组比较差异有统计学意义(P<0.01),而4∶1组的GVHD与对照组比较差异则无统计学意义(P>0.05)。结论:移植物内T细胞与受体循环血内的T细胞绝对值之比介于1∶1与2∶1之间较为合适;按此比例进行的大鼠移植,GVHD较轻。Objective To study the influence of T cells ratio between graft and recipient on graft-versus-host disease （GVHD） in rats. Methods In allogenic bone marrow transplantation （allo-BMT） model, 40 Sprague-Dawley rats were transplanted with the bone marrow from 40 Wistar rats. Recipient rats were divided into 4 groups according to the T cells ratio between graft and recipient, including 1：1, 2：1,4：1 groups and control group,and the occurrence and degree of GVHD after allogeneic peripheral blood stem cell transplantation （allo-PBSCT） were observed. Results The GVHD occurred in 1：1 group and in 2：1 group were the most mild and the differences between these two groups and control group were statistically significant （P〈0.01）. However, there was no significant difference between 4：1 group and control group （P〉0.05）. Conclusions The most appropriate T cell ratio between graft and recipient would be 1：1 or 2：1; the GVHD occurred after stem cell transplantation would be milder with these T cell ratio.

关 键 词：移植物-受体T细胞比例 移植物抗宿主反应 实验研究 

分 类 号：R457.7[医药卫生—治疗学] Q95-33[医药卫生—临床医学]