白细胞介素-12基因修饰对树突状细胞表面分子表达及细胞因子分泌的影响  

Effect of IL-12 gene modification on the phenotype and cytokine secretion of dendritic cells

作　　者：罗道升[1] 戴宇平[1] 马毅[2] 郑甫伏[1] 罗彬[1] 张珑娟[3] 赵继宗[3] 

机构地区：[1]中山大学附属第一医院泌尿外科,广州510080 [2]中山大学附属第一医院器官移植科,广州510080 [3]中山大学附属第一医院外科实验室,广州510080

出　　处：《中华实验外科杂志》2008年第3期354-356,共3页Chinese Journal of Experimental Surgery

摘　　要：目的观察白细胞介素（IL）-12基因修饰对树突状细胞（DC）表面分子及细胞因子分泌的影响。方法采用重组逆转录病毒介导IL-12基因修饰人外周血单个核细胞（PBMC）来源的DC；ELISA法检测各组DCs和各组T细胞上清中IL-12、IL-10、IFN-γ因子的分泌水平；流式细胞仪（FACS）分析各组DC表面CD83、CD86的表达；MTT法检测DC刺激同源T淋巴细胞增殖的能力；统计学分析比较各组间的差异。结果IL-12基因修饰使得DC高表达CD83和CD86分子，分泌高水平IL-12及IFN-γ，但对IL-10因子的分泌无明显影响，刺激同源T淋巴细胞增殖明显，诱导激活的T细胞上清中IFN-γ水平显著增高、IL-10分泌水平显著降低。结论经IL-12基因修饰后的DC表型成熟，分泌IL-12及IFN-γ的能力增强，对IL-10因子的分泌无影响，能抑制T细胞分泌IL-10因子,优化抗原提呈的微环境。Objective To investigate the effects of interleukin-12 gene modification on the phenotype and cytokine secretion of dendritic cells （DCs） in vitro. Methods DCs were derived from human peripheral blood mononuclear cells （hPBMCs） , modified by recombinant IL-12 retrovirus. The IL-12, IL- 10 and IFN-γ levels in culture supernatant of DCs and T lymphocytes were examined by ELISA. The expression of CD83 and CD86 molecules on DCs surface was detected by FACS. Proliferation capacity of activating autologous T lymphocytes was evaluated by MTT. Results After being modified by IL-12 gene, DCs produced high level of IL-12 and IFN-γ but had on effect on the production of IL-10, and increased the expression of CD83 and CD86 molecules. Proliferation of autologous T lymphocytes was also enhanced after being stimulated by DC vaccine. DC vaccine could induce and activate T lymphocytes to produce high level of IFN-γ,but suppress the production of IL-10. Conclusion After being modified by IL-12 gene, DCs showed more mature.

关 键 词：树突状细胞 白细胞介素-12 基因修饰 细胞因子 

分 类 号：R392[医药卫生—免疫学]