一个编码推定的钙依赖磷酸酶催化亚基的番茄全长cDNA序列的克隆及其表达分析  

Cloning and expression analysis of a tomato full-length cDNA encoding a putative catalytic subunit of calcineurin-like phosphatase

作　　者：张志新[1] 徐幼平[2] 曹文渊[1] 蔡新忠[1] 

机构地区：[1]浙江大学生物技术研究所,杭州310029 [2]浙江大学分析测试中心,杭州310029

出　　处：《植物病理学报》2008年第1期35-43,共9页Acta Phytopathologica Sinica

基　　金：国家自然科学基金资助项目(30671352);教育部霍英东高等院校青年教师基金项目(101032)

摘　　要：钙依赖磷酸酶(Calcineurin)作为Ca2+信号传感器中继因子对受Ca2+调节的生物学过程起重要调控作用。本研究克隆了一个番茄全长cDNA序列,该序列与钙依赖磷酸酶催化亚基(Calcineurin A,CNA)序列同源。推定的蛋白产物由315个氨基酸组成,明显短于非植物来源的该类蛋白。该蛋白含有钙依赖磷酸酶类金属磷酸酶中保守的催化作用活性位点基序,以及两个潜在的钙调蛋白结合位点,故暂时命名为LeCAL1(Lycopersicon esculentum calcineurin A like 1)。对CNA序列的分析结果表明,植物与非植物CNA差异明显,在系统进化树中形成距离很远的两大分支。植物CNA中,LeCAL1与拟南芥CNA同源性较高,而与水稻CNA较低。对LeCAL1在番茄中的表达分析结果显示,Cf/Avr介导的过敏性反应的产生诱导LeCAL1基因的表达,表明钙依赖磷酸酶可能在番茄抗叶霉病中起调节作用。Calcineurin as a Ca^2＋ sensor relay plays an important role in modulation of biological processes regulated by Ca^2＋. A tomato full-length cDNA, which was homologous to the sequences encoding a catalytic subunit of calcineurin （ Calcineurin A, CNA）, was cloned in the study. The deduced product, composed of 315 amino acids, was significantly shorter than non-plant CNA. It contained motifs conserved in CNA and two potential calmodulin-binding sites, thus was temporally named as LeCAL1 （Lycopersicon esculentum calcineurin A like 1 ）. Analysis of CNA sequence data revealed that plant and non-plant CNA differed significantly, and formed two evolutionally distanced clades. Among the known plant CNA, LeCAL1 is most similar to Arabidopsis CNA, while less homologous to rice CNA. Result of expression assay for LeCAL1 in tomato showed that Cf/Avr-dependent hypersensitive response induced LeCAL1 expression, indicating possible involvement of LeCAL1 in regulation of Cf-dependent resistance to tomato leaf mould disease.

关 键 词：LeCAL1 钙依赖磷酸酶(Calcineurin) 番茄 CLADOSPORIUM fulvum 过敏性反应 抗病性 

分 类 号：S432.4[农业科学—植物病理学]