大豆品种(系)抗疫霉根腐病基因的连锁SSR标记分析  

Analysis of Phytophthora resistance loci Rps1 and Rps4 in soybean cultivars or lines using linkage SSR markers

作　　者：陈晓玲[1,2] 朱振东[1] 杜青[1,2] 王晓鸣[1] 肖炎农[2] 武小菲[1] 

机构地区：[1]中国农业科学院作物科学研究所,国家农作物基因资源与基因改良重大科学工程,北京100081 [2]华中农业大学植物科技学院,武汉430070

出　　处：《植物病理学报》2008年第1期75-82,共8页Acta Phytopathologica Sinica

基　　金：国家自然科学基金(30370861,30170586);国家科技支撑计划课题(2006BAD09A08);农业行业科研专项(nyhyzx07-053)

摘　　要：大豆疫霉根腐病是大豆破坏性病害之一。利用抗病品种是防治该病的唯一有效的方法。迄今,已经鉴定了15个抗大豆疫霉根腐病基因(Rps基因),而且大豆部分基因都获得了不同类型的分子标记。本研究根据以前抗病基因推导结果选择26个可能含有Rps1基因座位等位基因或(和)Rps4基因的大豆品种(系),利用分别与Rps1a和Rps4紧密连锁的SSR标记进行抗病基因的分子检测,通过比较含有已知抗病基因对照大豆品种(系)分子标记检测结果并综合以往基因推导结果推断检测品种(系)的Rps基因。在选择的26个品种(系)中,长农14号被证明含有Rps1a,周豆13和铁95068-5含有Rps1a与Rps4基因组合,品系50794、科8924-3和合豆1号含有Rps4;有11个品种(系)存在含有Rps1a的证据、品系50052被推断含有Rps1c与Rps3b基因组合,但不排除这些品种(系)在Rps1座位含有一个新等位基因的可能性。另外,有7个品种(系)的所含抗病基因不能确定,它们可能含有新的Rps基因。Phytophthora root rot is a destructive disease of soybean. Growing resistant cultivars is the most effective way to control the disease. Up to now, 15 Phytophthora resistance genes （Rps gene） have been found, and most of them have been tagged and mapped by using molecular markers. In this study, 26 soybean cultivars or lines formerly postulated to carry alleles of Rpsl locus or （and） gene Rps4 were selected to detect Rps genes by using SSR markers linked tightly to Rpsla or Rps4. By comparison of SSR markers linked to known Rps alleles in control cultivars and in combination with the former results of gene postulation, the Rps genes in selected soybean cultivars or lines were proposed. Of 26 cultivars or lines, Changnongl4 was likely to possess Rpsla,Zhoudoul3 and Tie95068-5 possibly possessed gene combination of Rpsla and Rps4, line 50794, Ke8924-3 and Hedoul could carry Rps4. Rpsla was detected in 11 cultivars or lines,and line 50052 likely contained a gene combination of Rpslc and Rps3b,but it was not ruled out that these cultivars or lines possibly contained a new allele at the Rpsl locus. In addition, Rps genes in 8 cultivars or lines could not be determined and possibly contain some novel Rps genes.

关 键 词：大豆 疫霉根腐病 SSR标记 抗病基因 分子鉴定 

分 类 号：S432.21[农业科学—植物病理学]