流动注射-化学发光法定量测定同一水域中的腐殖酸  

Effect of Humic Acids on Determining Polyhydroxy Phenol by Flow-Injection Chemiluminescence

作　　者：丁保军[1] 杨凤林[1] 朱珍亮[2] 肖光[2] 崔丽钧[2] 

机构地区：[1]大连理工大学环境与生命学院,辽宁大连116024 [2]大连理工大学化学系,辽宁大连116024

出　　处：《光谱学与光谱分析》2008年第3期530-533,共4页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(50578023)资助

摘　　要：采用流动注射-化学发光(FI-CL)这一灵敏的检测方法,通过条件实验与参数优化,优选了测定环境中痕量多羟基酚类化合物的发光体系,通过考察天然水体中的腐殖酸对化学发光-多羟基酚测定体系的影响,为来自于水及土壤中的实际样品中腐殖酸的检测提供了参考,建立了同一区域水体中腐殖酸的化学发光定量测定方法。所得线性回归方程y=70·36x+540·1,相关系数r=0·9954,线性范围为3～15mg·L-1,检测限为0·749mg·L-1,HA浓度为6mg·L-1时的相对标准偏差(RSD)为1·08%。Flow-injection chemiluminescence was applied to determine the trace polyhydroxy phenol in environment（soil and water）.Based on the comparison of several different chemiluminescence systems and optimization,an alkaline luminol-NaIO4 system for determinating polyhydroxy phenol was chosen.During the process of polyhydroxy phenol determination,the effect of dissolved humic acid（HA） on the determination of phenol by this chemiluminescence system was also considered,which generally coexists in soil and water widely.Thus the present work provided the reference for the determination of the real samples coming from soil or water.An obvious restraining effect of humic acid on the chemiluminescence signal obtained by the luminol-NaIO4-polyhydroxy phenol system was found,which shows a linear relationship with the concentration of HA in a certain range,the linear equation is y=70.36x＋540.1,the correlation coefficient is 0.9954,the linear range is between 3-15 mg·L^-1,the limitation of detection is 0.749 mg·L^-1,and the relative standard deviation（RSD）is 1.08% when the concentration of HA is 6 mg·L^-1.The possible working mechanism of humic acid here is discussed and deduced.

关 键 词：流动注射-化学发光分析法 多羟基酚类化合物 腐殖酸(HA) 鲁米诺 

分 类 号：O482.3[理学—固体物理]