美洲商陆对锰毒生理响应的FTIR研究  

Studies on the Physiological Response of Phytolacca Americana to Manganese Toxicity by FTIR Spectroscopy

作　　者：任立民[1] 成则丰[1] 刘鹏[1] 李志刚[2] 

机构地区：[1]浙江师范大学植物学实验室,浙江金华321004 [2]广西大学农学院,广西南宁530004

出　　处：《光谱学与光谱分析》2008年第3期582-585,共4页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(30540056,40573052);浙江省自然科学基金项目(303461,304185,304186,305151,404135)资助

摘　　要：在一定Mn浓度梯度处理下(0,0·125,0·25,0·5,1g·kg-1),超积累植物——美洲商陆(Phyto-laccaamericana)不同组织器官的傅里叶变换红外光谱(FTIR)图谱发生了变化。其中,茎组织在3336和2916cm-1处峰高先上升后下降,反映了有机物运输受阻情况的变化,即美洲商陆在低Mn刺激下会产生大量有机物作为渗透调节物质来增强其耐Mn性,高Mn则抑制了有机物的分泌和运输;根和叶组织分别在2922和1606cm-1处表现不同变化趋势,但都反映了一个变化规律即低Mn处理下美洲商陆分泌的有机酸不断螯合Mn,随着Mn毒害的加重,其羧酸螯合力变弱;根组织1732和1026cm-1、茎组织1028cm-1、叶组织1052和967cm-1处呈现差异性变化,但都与其膜脂过氧化有关;根组织1375cm-1处峰高先上升后下降,可能与植物在细胞壁结构上增强抗逆性有一定关系,即低Mn处理下细胞壁可能通过阳离子交换能力(CEC)的提高增强了耐Mn性。以上说明,利用FTIR研究重金属超积累植物化学组分具有应用价值。A technique based on Fourier transform infrared（FTIR） spectrometry was used to detect the physiological changes in chemical composition of Phytolacca americana under a wide range of Mn^2＋ treatments（0,0.125,0.25,0.5 and 1 g·kg^-1）.Using the roots,stems and leaves of phytolacca americana,absorption bands corresponding to carbohydrates,ester and proteins varied differently.The absorption band heights at 3 000 and 2 916 cm^-1 of stem tissues rose firstly and then decreased,indicating the exudation and transporting situation of organic substances which served as organic osmotic contents to enhance manganese tolerance under low treatments;Meanwhile,the band heights at 2 922 cm^-1（roots） and 1 606 cm^-1（leaves） which have different tendency in accordance with Mn treatments showed that the capability to chelate Mn decreased under higher manganese concentration.In addition,the changes of the bands at 1732 and 1 026 cm^-1（roots）,1 028 cm^-1（stems）,1 052 and 967 cm^-1（leaves） differed from each other,suggesting that under the conditions of severe Mn stress the ex-oxidation of membrane lipid increased;Otherwise,the band heights at 1 375 cm^-1 increased firstly and then decreased,representing that the cell wall improved Mn resistance by increasing cation-exchange capacity（CEC）.The result also implied that it is practical to apply FTIR to the research comparing the chemical differences of phytoaccumulators under metal treatments.

关 键 词：傅里叶变换红外光谱 美洲商陆 Mn毒 化学组分 

分 类 号：O657.3[理学—分析化学]