小鼠白念珠菌性阴道炎局部组织中Th1/Th2因子的表达  

Local Th1/Th2 cytokine expression in experimental murine vaginal candidiasis

作　　者：陈善娟[1] 李少华[2] 刘志香[1] 吴艳[1] 李家文[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院皮肤性病科,武汉430022 [2]华中科技大学同济医学院附属协和医院眼科,武汉430022

出　　处：《中国麻风皮肤病杂志》2008年第3期170-172,共3页China Journal of Leprosy and Skin Diseases

基　　金：湖北省卫生厅科研基金资助项目(NO.JXIB048);杨森科学研究基金项目

摘　　要：目的:研究小鼠白念珠菌性阴道炎局部组织中Th1/Th2细胞因子的表达。方法:构建ICR小鼠白念珠菌性阴道炎模型,采用RT-PCR方法检测不同时间小鼠阴道局部组织中Th1/Th2细胞因子(IL-2/IL-4、IL-10、TGF-β1)的表达。结果:雌激素化组接种小鼠阴道局部组织中IL-2水平在早期显著升高,IL-4则轻度升高,但均很快开始下降。IL-10的水平在感染后期缓慢升高并保持在高水平,而TGF-β1在整个感染过程中均在高水平且高于其他因子。结论:IL-2在感染早期及IL-10在后期的高表达分别与其参与清除及维持白念珠菌感染有关。TGF-β1可能在阴道黏膜缺乏针对白念珠菌的明显细胞免疫时发挥重要作用。Objective： To detemfine the Th1/Th2 eytokine expressions in vaginal tissue of experimental murine infected with eandidiasis. Methods： ICR murine vaginal eandidiasis model was established. Non- estrogen- treated mice were used as controls. The level of Th1 （IL- 2）/Th2 （IL- 4, IL- 10, TGF-β1） eytokines in murine vaginal tissue was determined by RT- PCR. Results： In estrogen- treated mice, IL- 2 was kept at the high level and IL- 4 increased slightly during early infection. IL- 10 was increased and kept at high level during late infection while the level of TGF - β1 was kept at high level and was higher than other eytokines during whole infection. Conclusion： IL - 2 is associated with clearance of mucesal C. albicans, and IL - 10 is related to the susceptibility to the infection. TGF- β1 may play a predominant role when the virtual absence of changes in other Th - type cytokines during the infections.

关 键 词：白念珠菌 阴道灌洗 动物模型 Th1/Th2因子 

分 类 号：R711.31[医药卫生—妇产科学]