氯地滴眼液中氯霉素的含量测定  

Determination of content of chloramphenicol in chloramphenicol and dexamethasone eye drops

作　　者：张黎明[1] 杨友兰[1] 蔡长坤[1] 

机构地区：[1]上海市第十人民医院,上海200072

出　　处：《基层医学论坛》2008年第7期193-195,共3页The Medical Forum

摘　　要：目的建立一阶导数分光光度法测定氯地滴眼液中氯霉素的含量。方法一阶导数分光光度法,在305nm处测定氯地滴眼液的振幅D。结果线性范围为20.13￣30.01μg/ml,r=0.99989(n=5);平均回收率为99.78%,RSD=0.227%(n=5),氯地滴眼液中地塞米松及辅料无影响。结论该法操作简便、准确,消除了地塞米松的影响,适合于医院制剂氯地滴眼液中氯霉素的含量测定。Objective To establish a method for determination of content of chloramphenicol in chloramphenicol and dexamethasone eye drops by primary derivative spectrophotometry.Mcthocls Using primary＇ derivative spectrophotometry,the amplitude D of chloramphenicol and dexamethasone eye drops was determinated at the wavelength of 305 nm. Results linearity of chloramphenicol content was revealed over the range of 20.13-30.01 ug/ml,r=-0.99989 （n=5）.The average recovery was 99.78% with RSD of 0.227% （n=5）. Primary derivative spectrophotometry could eliminate the interference of dexamethasone and excipient.Conclusion It was a rapid,convenient and accurate method and eliminate the interference of dexamethasone.It could be fit for the determination of content of chloramphenicol in chloramphenicol and dexamethasone eye drops in hospital.

关 键 词：氯霉素 一阶导数分光光度法 含量测定 氯霉素地塞米松滴眼液 

分 类 号：R927[医药卫生—药学]