补骨脂与双氢青蒿素合剂治疗小鼠隐孢子虫病的实验研究  

Experimental Treatment Using Combined Fructus psoraleae and Dihydroartemisinin in Mouse Cryptosporidiosis

作　　者：陈玉凤[1] 秦元华[1] 郑莉莉[1] 戴晓冬[1] 任一鑫[1] 聂大平[2] 崔昱[1] 

机构地区：[1]大连医科大学寄生虫学教研室,大连116044 [2]大连医科大学附属二院检验科,大连116023

出　　处：《中国寄生虫学与寄生虫病杂志》2008年第1期67-69,共3页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：辽宁省教育厅科学研究计划资助项目(No.2004D103)~~

摘　　要：用补骨脂和双氢青蒿素合剂治疗经地塞米松诱导的隐孢子虫病小鼠,剂量为补骨脂3.4g/kg,双氢青蒿素60mg/(kg·d),灌胃,连用7d。结果粪检隐孢子虫卵囊数、全血CD4+、CD3+T细胞比例、血清γ干扰素(IFN-γ)浓度,以及小肠组织匀浆一氧化氮(NO)浓度均高于感染对照组(P值均<0.01)。小肠组织病变程度均轻于感染对照组。表明补骨脂和双氢青蒿素合剂可通过调节血清IFN-γ、全血CD4+、CD3+T细胞比例,以及提高肠组织NO浓度,参与宿主免疫应答和炎症反应过程,抑杀虫体、治疗小鼠隐孢子虫病。A combination of Fructus psoraleae （3.4 g/kg） and dihydroartemisinin （60mg/（kg.d）） was used for 7 d in mice infected with Cryptosporidium parvum infection induced by oral administration of dexamethasone. The results indicated that number of Cryptosporidium parvum oocysts in feces, proportion of CD^4＋, CD^3＋ T lymphocytes in blood, serum IFN-γ and nitric oxide （NO） content in intestinal tissue were all higher than those of infected control group （P〈0.01）. The pathogenic change in intestine of treated mice was lighter than that of infected control. It is indicates that the combination of Fructus psoraleae and dihydroartemisinin is effective to mice cryptosporidiosis probably through upregulating serum IFN-γ proportion of CD^4＋ and CD^3＋ T cells and NO concentration in intestine.

关 键 词：补骨脂 双氢青蒿素 隐孢子虫病 Γ干扰素 T细胞 一氧化氮 

分 类 号：R531.5[医药卫生—内科学]