旋毛虫对昆明小鼠最小感染剂量的实验研究  

Minimum Dose of Trichinella spiralis Muscle Larvae to Infect Mice

作　　者：崔晶[1] 王洁[1] 王中全[1] 牛洪涛[1] 

机构地区：[1]郑州大学医学院寄生虫学教研室,郑州450052

出　　处：《中国寄生虫学与寄生虫病杂志》2008年第1期73-74,共2页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：河南省杰出人才创新基金(No.0321001900);河南省高校杰出科研人才创新工程项目(No.2004KYCX013)~~

摘　　要：为观察旋毛虫感染昆明小鼠的最小剂量,将70只雄性昆明小鼠随机均分7组,每只鼠分别经口感染旋毛虫肌幼虫30、25、20、15、10、5及3条,感染后6周剖杀。取完整膈肌,压片、镜检、计数虫荷。小鼠全身肌肉经人工胃液消化后计数肌肉虫荷。结果,压片镜检法和人工消化法的幼虫检出率,感染30、25、20、15及10条旋毛虫肌幼虫的小鼠均为100%(10/10);感染5条旋毛虫肌幼虫的小鼠,两种方法幼虫检出率分别为70%(7/10)和100%(10/10);感染3条旋毛虫肌幼虫的小鼠,两种方法均未检出幼虫。感染旋毛虫的小鼠膈肌和肌肉虫荷与感染剂量均呈正相关(r=0.759,P<0.05;r=0.638,P<0.05)。旋毛虫经口成功感染昆明小鼠的最小剂量为5条幼虫。Seventy male mice （Kunming strain） were randomly divided into 7 groups（10 mice per group）, each mouse was orally inoculated with 30, 25, 20, 15, 10, 5 or 3 muscle stage larvae of Trichinella spiralis, respectively. All infected mice were sacrificed 6 weeks post-inoculation, number of larvae per gram （LPG） of diaphragm were counted by compression method （trichinelloscopy）, the carcass was digested by artificial digestion method and LPG was counted. The larval detection rate by trichinelloscopy and digestion method was 100%（10/10） in all mice infected with 30, 25, 20, 15 or 10 larvae, but 70% （7/10） and 100% in mice infected with 5 larvae, respectively. No larva was found by either method in mice infected with 3 larvae. There is a positive correlation between the larval burden （of diaphragm and muscle） and the infecting dose （r=0.759, P〈0.05; r=0.638, P〈0.05）, and the minimum infection dose to a mouse is 5 larvae.

关 键 词：旋毛虫 感染剂量 昆明小鼠 

分 类 号：R383.15[医药卫生—医学寄生虫学]