肠出血性大肠杆菌O157:H7噬菌体的分离纯化  

Separation and Purification of Enterohemorrhagic E. coli O157:H7 Bacteriophage

作　　者：杜崇涛[1] 王文东[1] 刘军[2] 孙洋[2] 齐翀[2] 祝令伟[2] 郭学军[2] 冯书章[2] 

机构地区：[1]吉林大学农学部畜牧兽医学院,吉林长春130062 [2]军事医学科学院军事兽医研究所,吉林长春130062

出　　处：《吉林畜牧兽医》2008年第4期6-8,共3页Jilin Animal Husbandry and Veterinary Medicine

基　　金：国家"973"基金资助项目(2006CB504400)

摘　　要：分离纯化了抑制肠出血性大肠杆菌(Enterohemorrhagic E.coli,EHEC)O157:H7的噬菌体,并对其进行效价测定。将宿主菌EHEC O157:H7培养制成悬液后与污水样品37℃共培养16h,将培养物离心、过滤除菌后得到该菌的噬菌体原液。噬菌体原液采用双层琼脂平板法进行5次纯化,得到直径相同的噬菌斑。纯化后噬菌体的效价达到1.2×1010pfu。电镜观察表明,该噬菌体呈蝌蚪状。根据ICTV对病毒的分类标准,该噬菌体属长尾噬菌体科,为烈性噬菌体。The separation and purification of bacteriophage inhibiting Enterohemorrhagic E. coli O 157：H7 were studied and the titre of the isolated bacteriophage was evaluated. The host strain, EHEC O 157：H7, was cultured as a suspension and then cocultured with sewage at 37 ℃ for 16h, the cocultured sample was then centrifuged and filtered to eliminate bacteria and thus the bacteriophage superuatant was obtained. The bacteriophage superuatant was purified for 5 times using the two layer plates, and the bacteriophage plaques of uniform diameter were obtained. The titer of purified bac- tiriophage was 1.2 × 10^10pf u .The bacteriophage was tadpole-like bacteriophage as observed by electronmicroscope. According to ICIV（Interuational Committee for Taxonomy of Viruses）, it belongs to the Siphoviridae family and is a virulent phage.

关 键 词：O157:H7:噬菌体 分离 纯化 

分 类 号：S852.6[农业科学—基础兽医学]