异氟醚麻醉对大鼠海马蛋白质组的影响  

Effects of isoflurane on hippocampal proteome on the hippocnmpus proteome after in rats

作　　者：段开明[1] 欧阳文[1] 夏月峰[1] 张文静[1] 李冠[1] 

机构地区：[1]中南大学湘雅三医院麻醉科,长沙市410013

出　　处：《临床麻醉学杂志》2008年第3期239-241,共3页Journal of Clinical Anesthesiology

基　　金：国家自然科学基金(编号30771052);湖南省自然科学基金(编号07555039)

摘　　要：目的研究异氟醚麻醉后大鼠海马蛋白质组的变化。方法将10只8月龄SD大鼠随机均分为异氟醚组(I组)和对照组(C组)。I组大鼠以1·2%异氟醚吸入麻醉2h,C组吸氧对照。于麻醉处理或吸氧对照结束后24h取海马组织行双向凝胶电泳和质谱分析以鉴定差异蛋白点。结果双向凝胶电泳结果显示I和C组差异蛋白质点共有17(4/13)个,MALDI-TOF-MS分析后鉴定出12种蛋白质。结论异氟醚麻醉可以引起大鼠海马蛋白质组变化,这些蛋白质变化可能具有一定的神经元保护作用。Objective To investigate the alteration of hippocampus proteome after isoflurane anesthesia in rats. Methods Ten 8-month-old SD rats were randomly divided into control group（group C）and isofluroame group（group I）. The rats in group C only inhaled mixed gas containing 40% oxygen for two hours. The rats in group I were inhaled 1.2% isoflurane in 40% oxygen for two hours. The hippocampus of the rat was cut down for two dimensional gel electrophoresis （2-DE） 24 h after anesthesia. The different protein spots were analyzed by means of mass chromatographic analysis. Results There were 17 （4/13）different protein spots between group I and group C, of which 12 protein spots of all spots were identified by MALDI-TOF-MS. Conclusion The study shows that isoflurane may cause the alteration of hippocampal proteome in rats,and this change may be related to the neuron protection of isoflurane preconditioning.

关 键 词：异氟醚 大鼠 海马 蛋白质组 

分 类 号：R614[医药卫生—麻醉学]