银屑病患者造血细胞定向分化的CD4^＋CD25^＋调节性T细胞活性研究  

Functional characterization of CD4^+ CD25^+ regulatory T cells differentiated in vitro from bone marrow-derived hematopoietic cells of psoriatic patients

作　　者：董峰 李新华[2] 张开明[2] 尹国华[2] 

机构地区：[1]长治市第二人民医院皮肤科,山西长治046000 [2]太原市中心医院皮肤科,山西太原030009

出　　处：《临床皮肤科杂志》2008年第4期207-209,共3页Journal of Clinical Dermatology

基　　金：国家自然科学基金(30771940);山西省自然科学基金(20031110);太原市科技局基金(0503044)资助项目

摘　　要：目的:探讨造血细胞与银屑病患者CD4+ CD25+调节性T细胞活性异常的关系。方法:在胸腺基质细胞支持下,将骨髓CD34+细胞向T细胞定向分化,免疫磁珠法分离CD4+ CD25+调节性T细胞,分别在自然增殖状态及链球菌超抗原刺激下,采用四甲基偶氮唑蓝(MTT)法和ELISA法测定定向分化的CD4+ CD25+ T细胞增殖活性及分泌细胞因子水平。结果:在自然增殖状态下,银屑病患者CD34+细胞定向分化的CD4+ CD25+ T细胞显示与正常对照相似的增殖活性及白介素(IL)-2、IL-4、IL-8、IL-10、干扰素(IFN)-γ等细胞因子分泌水平,然而,在受到A族链球菌超抗原刺激后,银屑病患者定向分化的CD4+ CD25+ T细胞较正常对照显示相对低的增殖活性及分泌IL-2、IL-10能力。结论:银屑病患者CD34+细胞定向分化的CD4+ CD25+ T细胞功能异常,提示银屑病患者调节性T细胞功能异常可能源自遗传背景决定的骨髓造血细胞活性。Objectives： To confirm the hypothesis that hematopoietic stem cells are responsible for dysfunctional CD4^＋CD25^＋ regulatory cells in psoriasis. Methods： Bone marrow-derived CD34^＋ hematopoietic cells from psoriatic patients with family history and normal control were differentiated to T cells in vitro. CD4^＋CD25^＋ T cells were isolated by immunomagnetic beads, and furthermore proliferation activity and capacity of secreting cytokines were determined by MTT and ELISA. Results： The differentiated CD4^＋CD25^＋ T cells of psoriatic origin showed similar characteristics to those of normal volunteers, including pro- liferation, secreting profile of cytokines （IL-2, IL-4, IL-8, IL-10 and IFN-γ）. However, proliferation and levels of cytokines IL-2, IL-10 from CD4^＋CD25^＋ cells of psoriatic CD34^＋ cells origin were significantly lower than those of normal control in response to Strep-A. Conclusions： CD4^＋CD25^＋ T cells differentiated in vitro from hematopoietic cells of psoriasis patients are impaired in the regulatory functions. It suggests the dysfunction of psoriatic CD4^＋CD25^＋ T cells due to an inherent genetic programming passed down from bone marrow hematopoietic cells.

关 键 词：银屑病 CD34^+细胞 造血细胞 T淋巴细胞 调节性T细胞 

分 类 号：R758.63[医药卫生—皮肤病学与性病学]