利用外源性IgG显示大鼠脊髓撞击伤后血-脊髓屏障变化  

Changes of the blood-spinal cord barrier following spinal cord contusion in rats detected by injection of exogenous IgG

作　　者：葛冠群[1] 沈学锋[1] 龚雪鹏[2] 赵宇[1] 王子田[1] 张欲凯[1] 游思维[1] 鞠躬[1] 

机构地区：[1]第四军医大学全军神经科学研究所,西安710032 [2]解放军451医院医学影像科,西安710054

出　　处：《神经解剖学杂志》2008年第2期119-122,共4页Chinese Journal of Neuroanatomy

基　　金：国家重点基础研究计划(2003CB515301);陕西省社发攻关项目(2006K15-G1)资助

摘　　要：血-脑(脊髓)屏障损伤范围的观察方法多为注射外源性示踪剂,我们曾在大鼠实验中采用内源性血浆IgG作为示踪剂,取得很好的效果。本研究在大鼠脊髓撞击伤模型中以静脉注射的兔IgG为外源性示踪剂,用免疫荧光双标显示并比较了大鼠与兔两种IgG在脊髓内的分布。用图像阈值分割技术处理图像后,分析了损伤后不同时间点(0h,1d,3d,5d,7d,14d)两者的异同,发现在血-脊髓屏障已恢复的部位仍有残留的内源性IgG,而外源性IgG可精确地反映注射当时血-脊髓屏障的改变。这种方法可有效地用于诸如不同药物保护血-脑屏障的时程研究。Abstract The area of injured bloed-brain （spinal cord） barrier （BBB, BSCB） is often detected by injection of exogenous tracers. We had successfully made use of the endogenous immunoglobulin G （IgG） as an indicator in rat experiments. In this study we injected rabbit IgG as an exogenous indicator and compared its distribution with that of the endogenous rat IgG with double immunostaining, and threshold image segmentation at0 h, 1 d, 5 d, 7 d, and 14 d after operation. We found that in areas where the enhanced BSCB had recovered, there was still remaining endogenous IgG, whereas the exogenous IgG accurately depicted the area of injured BSCB. This method can be used, for instance, to investigate the time course of drugs that may protect the BBB.

关 键 词：脊髓损伤 血-脊髓屏障 免疫球蛋白 

分 类 号：R651.2[医药卫生—外科学]