异丙酚麻醉对小鼠脑内ERK1／2磷酸化的影响  

Effect of propofol anesthesia on the expression of phosphorylated ERK1/2 in the mouse brain

作　　者：李刚[1] 成丹丹[1] 张西京[1] 朱正华[1] 段小莉[2] 王百忍[2] 熊利泽[1] 

机构地区：[1]第四军医大学西京医院麻醉科,西安710032 [2]第四军医大学神经科学研究所,西安710032

出　　处：《神经解剖学杂志》2008年第2期141-146,共6页Chinese Journal of Neuroanatomy

基　　金：国家自然科学基金(Nos.30471663,30600582)资助项目

摘　　要：为探索全身麻醉的中枢作用靶位,本研究观察了异丙酚麻醉-清醒过程中小鼠脑内磷酸化的细胞外信号调节激酶(pERK1/2)的表达变化。24只雄性BALB/c小鼠,随机分为4组:未麻醉对照组,异丙酚麻醉5min组,异丙酚麻醉后清醒5min组和异丙酚麻醉后清醒1h组;每组6只。麻醉动物腹腔内注射异丙酚100mg/kg。分别于清醒状态和麻醉各时点脱臼处死,灌注固定,取脑,冠状位冰冻切片,行全脑pERK1/2免疫组织化学染色,观察分析。结果显示:与未麻醉对照组相比,异丙酚麻醉5min组小鼠脑内感觉运动皮质、扣带皮质、梨状皮质、嗅周皮质及海马的pERK1/2阳性细胞数明显减少(P<0.05),而杏仁中央核、终纹床核卵圆亚核、下丘脑室旁核、视上核、下丘脑腹内侧核腹外侧部、缰外侧核和中脑E-W核等均较未麻醉组显著升高(P<0.01)。清醒5min后与麻醉组相比,各脑区/核团的pERK1/2表达除海马外均明显恢复(P<0.05);清醒1h后除海马外各脑区/核团的pERK1/2表达均恢复至对照组水平(P>0.05)。本研究表明异丙酚能引起小鼠脑内一些脑区和核团的ERK1/2磷酸化水平随麻醉过程发生变化,提示ERK1/2磷酸化可能参与全身麻醉的作用过程。相关脑区和部位可能与全麻药的中枢作用靶位或麻醉的应激效应调控有关。In order to screen the targets of general anesthetics in the central nervous system, we investigated the changes of phosphorylated extracellular signal regulated kinase （pERK1/2） expression in the mouse brain during the process of pmpofol anesthesia-emerging. Twenty-four male BALB/c mice were randomly divided into4 groups （n =6 for each group） ： a control group （awake mice without being anesthetized to serve as a control） and 3 pmpofol anesthesia groups including anesthetized for 5 min, awaken for 5 min from anesthesia, and awaken for I h from anesthesia. Anesthesia was induced by injection of 100 mg/kg pmpofol, i.p. All mice were sacrificed by cervical dislocation at corresponding time point and then perfused with 4% paraformaldehyde via the heart. The brains were removed and coronal sections of the brains were cut transversely on a cryostat and processed for immunohistechemical staining of pERK1/2. The results showed that the number of pERKl/2-immunoreactive cells in scnsorimotor cortex, cingulate cortex, piriform cortex, perirhinal cortex and hippoeampus was decreased significantly 5 min following propofol anesthesia （P 〈0.05）, while the number of positive cells in some subcortical nuclei including central amygdaloid nucleus, oval subnucleus of bed nucleus stria termimalis, hypothalamic paraventricular nucleus, supraoptic nucleus, ventrolateral portion of ventromedial hypothalamic nucleus, lateral habenular nucleus and Edinger-Westphal nucleus （E-W） was increased markedly at the same time, compared with that in control group （P 〈0.01 ）. Compared with that in the anesthesia group, the expression levels of pERK1/2 in the brain areas/nuclei except hippocampus were markedly restored 5 min after the mice awoke from anes- thesia （P 〈0.05 ）. The expression levels of pERK1/2 in the brain areas/nuclei except hippoeampus restored to the level of the controlgroup 1 h after the animals emerged from the anesthcsia （P〉0.05）. The present results suggest that propofol ancsthesia induc

关 键 词：异丙酚 全身麻醉 细胞外信号调节激酶 磷酸化 脑 小鼠 

分 类 号：R614.2[医药卫生—麻醉学]