应用穿孔全细胞膜片钳记录技术研究培养大鼠耳蜗螺旋神经节神经元的基本电生理特性  

Electrophysiological properties cultured in the rat spiral ganglion neurons examined with perforated whole cell patch-clamp recording technique

作　　者：查定军[1] 王智明[2] 薛涛[1] 林颖[1] 邱建华[1] 李云庆[2] 

机构地区：[1]第四军医大学西京医院全军耳鼻咽喉-头颈外科中心,西安710032 [2]第四军医大学基础部解剖学教研室、粱銶琚脑研究中心,西安710032

出　　处：《神经解剖学杂志》2008年第2期157-160,共4页Chinese Journal of Neuroanatomy

基　　金：国家自然科学基金(Nos.30771133,30370458,30600702);陕西省自然科学基金(No.2003C2051)资助项目

摘　　要：为了深入了解耳蜗螺旋神经节(SG)神经元的基本电生理特点,本研究首先成功培养了急性分离的大鼠SG神经元,然后采用穿孔全细胞膜片钳记录技术观察了培养SG神经元的基本电生理特性。结果表明,可从培养的SG神经元记录到动作电位,且不同的SG神经元具有异质性的放电特征。上述结果提示穿孔全细胞膜片钳记录技术可用于体外培养耳蜗SG神经元的电生理特征研究,为开展听觉信息传导和调控的功能研究开辟了新途径。In order to further investigate the electrophysiological properties of cochlea spiral ganglion （SG） neurons, at first, the cell culture method for acutely dissociated SG neurons from SD rats （postnatal 0 -5 d old） was successfully established. Then, the basic electrophysiological properties of the cultured SG neurons were examined with perforated whole cell patch-clamp recording technique in the present study. The present results showed that action potentials could be recorded from the cultured SG neurons and different SG neurons exhibited a variety of firing characters about their action potentials. The present results suggest that： （1） Pedorated whole cell pateh-clamp recording method can be used for examining the electrophysiolngical properties of the cultured SG neurons; （2） For the functional analysis of the hearing information transformation and regulation, perforated whole cell patch-clamp recording method might create a new approaching pathway.

关 键 词：螺旋神经节神经元 细胞培养 穿孔全细胞膜片钳记录 大鼠 

分 类 号：R33[医药卫生—人体生理学]