利用替代寄主生产马尾松毛虫质型多角体病毒的研究  

SUBSTITUTIONAL HOST USED FOR PRODUCTION OF DpCPV

作　　者：曾陈湘[1] 吴若光[1] 何雪香[1] 何立平[1] 谢诚 刘德胡 陈天来 

机构地区：[1]广东省林业科学研究院 [2]惠州市林业局

出　　处：《广东林业科技》1997年第4期6-13,共8页Forestry Science and Technology of Guangdong Province

摘　　要：ＤｐＣＰＶ对８种鳞翅目昆虫感染试验结果表明，棉铃虫最敏感，感染率达９７．０％。ＤｐＣＰＶ对棉铃虫的毒力ＬＣ５０＝７．６５×１０４ＣＰＢ／ｍｌ，浓度与感染死亡率相关性及回归方程均达到显著性。ＨａＤｐＣＰＶ与原毒种ＤｐＣＰＶ的形态特征相同；棉铃虫可用人工半合成饲料配方２在小塑料盒内单虫饲养，幼虫成活率达９８％以上、羽化率达９０％，平均蛹重０．４００６ｇ，平均产卵量７９７．７粒，卵孵化率达９８．２％；ＨａＤｐＣＰＶ对第一代４～５龄马尾松毛虫的毒力较强，ＬＣ５０＝１．１４×１０４ＣＰＢ／ｍｌ，与原毒种相比，毒力没有下降。用棉铃虫来生产ＤｐＣＰＶ，采用２３～２６℃、３龄虫、使用浓度１０５ＣＰＢ／ｍｌ以上，是比较理想的组合。将病毒喷在人工饲料表面喂虫，可获得较高的病毒产量，平均每条虫可产８．１３亿ＣＰＢ，与用原宿主生产的产量相接近。用ＨａＤｐＣＰＶ防治第一、二代４～５龄马尾松毛虫，防治效果达６０．２％～８４．０％。species of Lepidoptera insects were treated with DpCPV and the result showed that Heliothis armigera was the most susceptible to DpCPV with the infected rate up to 97%. The median lethal concentration of DpCPV to Haliothis armigera was 7.65×10 4 CPB/ml, the correlativity between concentration and infected lethal rate and their regression equation were significant. In addition, the morphological characteristic between Ha DpCPV and the original DpCPV was the same. Heliothis armigera was individually raised in a small plastic container with semiartificial feed, No.2 prescription. Larva survival rate was more than 98%, eclosion rate was 90%, averaged weight of pupa was 0.4006 g,averaged output of eggs was 797.7,incubating rate was 98.2%. Infected rate of Heliothis armigera inoculated with DpCPV was 81.0%. Ha DpCPV was the most virulent to the 4～5 instar moths of the first and second generation, and its virulence was not decreased when compared to that of the original DpCPV. It was favourable to produce DpCPV at the temperature of 23～26℃, inoculation concentration of over 10 5 CPB/ml, with 3 instar Heliothis armigera . The output of Ha DpCPV from Heliothis armigera was averaged 8.125×10 8 CPB per head, same as that from Dendrolimus punctatus . To get more Ha DpCPV, the inoculum should be sprayed on the surface of feed for Heliothis armigera . Ha DpCPV was used to supress 4～5 instar moths of the second generation, the control effectiveness reached 60.2%～84.0% in forest field.

关 键 词：多角体病毒 替代寄主 马尾松毛虫 棉铃虫 

分 类 号：S763.420.6[农业科学—森林保护学] S763.853[农业科学—林学]