抗戊型肝炎病毒单克隆抗体识别表位的初步研究  

The Preliminary Analysis of the Recognition Epitopes of Anti-HEV Monoclonal Antibodies on HEV ORF2

作　　者：熊君辉[1] 郭清顺[1] 葛胜祥[1] 顾颖[1] 陈毅歆[1] 苗季[1] 杜海莲[1] 史维国[1] 张军[1] 夏宁邵[1] 

机构地区：[1]厦门大学国家传染病诊断试剂与疫苗工程技术研究中心,厦门大学生命科学学院,福建厦门361005

出　　处：《病毒学报》2008年第2期83-87,共5页Chinese Journal of Virology

基　　金：863计划(2005DFA30820);国家自然科学基金(30500092);国家自然科学基金(30600106);福建省科技重大专项(2004YZ01-1);福建省科技创新项目(2006F3124)

摘　　要：通过Western blot、体外捕获PCR、ELISA阻断实验及合成的多肽库等方法，对23株抗戊型肝炎病毒（HEV）单克隆抗体（单抗）识别HEVORF2表位的作用进行系统研究。结果显示，7株线性单抗识别表位都位于ORF2aa408-458之间，16株构象型单抗识别表位都定位于ORF2aa459-606之间，大部分构象型单抗识别表位都在天然病毒表面。对这些单抗识别表位系统地了解将为HEV疫苗、诊断、病毒受体和病毒感染机制等方面的研究提供重要工具。Western blot, capture-PCR, blocking ELISA and synthetic polypeptides were used to systemati- cally study the recognition epitopes on HEV ORF2 of 23 anti-HEV monoclonal antibodies（McAbs） which were previously generated in our laboratory directed against HEV ORF2. Results showed that seven McAbs recognized linear epitopes that located at aa408-458 of HEV ORF2 and 16 conformation-dependent McAbs, most of which recognized the surface epitopes of native HEV, located at aa459-606 of HEV ORF2. The systematical study of the recognition epitopes of anti-HEV McAbs on HEV ORF2 provides important information for the investigation of virus receptor and HEV infection mechanism, as well as its vaccine and diagnostics development.

关 键 词：戊型肝炎病毒(HEV) 单克隆抗体(单抗 McAb) 表位 

分 类 号：R373[医药卫生—病原生物学] Q78[医药卫生—基础医学]