甘肃省苜蓿种质资源遗传多样性RAPD分析  

RAPD Analysis of Genetic Diversity of Alfalfa Germplasms in Gansu Province

作　　者：杨晓莉[1] 陈丽[2] 班霆[1] 韩鹏[1] 王晓娟[1] 

机构地区：[1]兰州大学草地农业科技学院 [2]甘肃省草原生态研究所,甘肃兰州730020

出　　处：《草地学报》2008年第2期129-134,共6页Acta Agrestia Sinica

基　　金：国家重点研究发展计划项目(2007CB108904);甘肃省自然科学基金项目(3ZS051-A25-066);兰州大学引进人才专项基金项目(582402)

摘　　要：苜蓿(Medicago sativa L.)是世界上重要的豆科牧草,了解栽培品种的遗传关系和遗传距离对于育种工作具有重要意义。本研究利用RAPD标记技术对甘肃省11个苜蓿栽培品种(群)进行遗传多样性分析,以期为进一步开展苜蓿新品种选育奠定基础。结果表明:10个随机引物共检测到132条谱带,其中107条为多态性带,多态性条带比率(PPB)高达81.1%;天水苜蓿和定西苜蓿之间的Roger’s遗传距离较远(0.4610),而与陇中苜蓿之间的遗传距离较近(0.1406);进一步的AMOVA分析显示苜蓿种群大部分的遗传变异发生在种群内(61.25%),有38.75%的遗传变异发生在种群间,种群分化不明显。Alfalfa （Medicago sativa L. ） is a forage legume of world-wide importance and the genetic relationship and distance among cultivars are of great interest for breeding programs. In this paper, random amplified polymorphic DNA （RAPD） was used to analyze the genetic diversity of 11 alfalfa cultivars. A total of 132 discernible loci were obtained for all populations using 10 primers, and 81.1% of these loci were polymorphic, which indicated a high genetic diversity in the cultivars from Gansu Province. According to the Roger＇s distance, the smallest MRD （0. 1406） occurred between cultivars ‘ Tianshui＇ and ‘Longzhong＇, while the largest （0. 4610） was detected between cultivars ‘Tianshui＇ and ‘Dingxi＇. The result of AMOVA analysis showed that the majority （61.25%） of genetic variation was within populations and 38. 75% among populations.

关 键 词：苜蓿 种质资源 遗传多样性 RAPD 

分 类 号：S812[农业科学—草业科学] Q943[农业科学—畜牧学]