新型微波天线热凝大鼠骨骼肌的变化  

Rat skeletal muscle alterations after coagulation with novel microwave antenna

作　　者：何金霞[1] 张俊德[1] 李虹义[1] 张积仁[1] 

机构地区：[1]南方医科大学珠江医院肿瘤中心,广东省广州市510282

出　　处：《中国组织工程研究与临床康复》2008年第9期1753-1756,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：目的:观察一种新型微波天线热凝大鼠骨骼肌后的病理生理改变,认识新型微波天线应用于体表肿瘤及微小肿瘤的安全性及可行性。方法:实验于2006-12/2007-09在南方医科大学珠江医院中心实验室完成。①微波热凝:应用MTC-3C组织间肿瘤微波凝固治疗仪,直视下将经防粘连处理的单极辐射微波天线垂直插入SD大鼠骨骼肌,采用20W×8s功率时间组合热凝。②观察指标:TTC染色法观察热凝后即刻酶活性变化,流式细胞仪检测凝固后0,12,24h热凝区外周边缘组织细胞的凋亡率,苏木精-伊红染色观察热凝后0,2,7,30,60d时组织学变化。结果:①TTC染色结果:热凝区呈苍白色,周围正常组织为鲜红色。边缘组织细胞在热凝后即刻出现凋亡,12h时凋亡率最高,随后逐渐下降。②苏木精-伊红染色结果:热凝后即刻细胞形态无明显变化;2d后过渡区出现大量坏死细胞,大量炎性细胞浸润,热凝区细胞外形仍维持;7d时过渡区纤维母细胞、肉芽组织形成,新生胶原沉积,中央热凝区肌纤维逐渐溶解、细胞坏死;30d后热凝区慢性炎症反应明显,过渡区一些胶原纤维生成;60d后固化坏死细胞减少,过渡区大量胶原纤维生成,组织的修复是从外周过渡区开始逐渐向中央热凝区进行。结论:新型微波天线用于体表肿瘤及微小肿瘤的治疗是安全可行的。AIM： To evaluate the feasibility and safety of microwave coagulation with novel microwave antenna through observing physiological and pathological changes alter the coagulation on rat skeletal muscle. METHODS： The experiment was performed at the Central Laboratory of Zhujiang Hospital of Southern Medical University from December 2006 and September 2007. （1）Using MTC-3C microwave tissue coagulation device, monopolc microwave antenna alter anti-adhesion was vertically inserted into SD rat skeletal muscle for heat coagulation at 20 W×8 s. （2）The instant enzyme activity changes after microwave coagulation therapy （MCT） was detected by 2, 3, 5-Triphenyl Tetrazolium Chloride （TTC） staining. The cell apoptosis rate in outer fields of coagulation was evaluated by flow cytometry 0, 12 and 24 hours after coagulation, and the trealed muscle was examined patbologically on days 0, 2, 7, 30, and 60. RESULTS： （1）Thc coagulation areas were stained in white immediately alter MCT by TTC staining, while the outer normal areas were in red. There were apoptotic cells around coagulation areas, and the highest percentage appeared 12 hours later, reduced thereafter.（ 21）In 24 hours after coagulation, only mild alteration of cellular morphology was found. Two days after MCT, necrosis cells were prominent in transition zone, and the inflammation cells were predominantly composed of macrophage and lympbocytes in the zone, while the cells in the coagulation zone remained structure： 7 days after MCT, the fibroblast and granulation tissue occurred in the transition zone. and the myocyte in the coagulation zone were of necrosis： 30 days after MCT, there were still many inflammation cells in the coagulation zone with some collagenous fibrils in the trunsition zone： 60 days after MCT, the necrosis cells reduced and the outer margin of the transition zone was encapsulated by marked collagenous fibrils. Tissue repair started from the outer margin of the transition zone to the central coagulation zone. CONCL

关 键 词：微波凝固 病理变化 细胞凋亡 医学工程学 

分 类 号：R318[医药卫生—生物医学工程]