心室间隔膜部及嵴内型缺损动物模型的建立(英文)  

Animal models of intracristal and membranous ventricular septal defect

作　　者：周达新[1] 管丽华[1] 王晓彦[1] 王宜青[1] 黄国倩[1] 葛均波[1] 诸骏仁[1] 

机构地区：[1]复旦大学附属中山医院心内科,上海市200032

出　　处：《中国组织工程研究与临床康复》2008年第9期1757-1760,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：上海市科委资助项目(034119903)~~

摘　　要：背景:膜部和嵴内型室间隔缺损周围结构比较复杂,进行封堵治疗存在一定的难度,因此确定最佳干预方法需要建立相应的动物模型。目的:在超声心动图定位下建立室间隔膜部及嵴内型缺损动物模型。设计:动物造模实验。单位:复旦大学附属中山医院心内科。材料:选用健康成年草狗6只,雌雄不拘,从上海市实验动物中心购置。实验用主动脉-大隐静脉近端吻合器-打孔器为美国St.Jude公司产品,并对其进行改良即将其头部的塑料外壳成分削平、薄,也可把塑料外壳削短,使通过荷包进右室穿刺室间隔时阻力减少。Philips Sonos 5500多功能超声诊断仪由美国菲利普公司生产,经胸超声探头频率为2.5～3.5MHz。方法:实验于2006-04/07在复旦大学附属中山医院、上海市心血管病研究所中心实验室完成(生物级别:BSL2级)。将6只草狗麻醉后,取卧位,在超声心动图定位下,使用主动脉-大隐静脉近端吻合器-打孔器对室间隔穿刺,制备室间隔缺损。实验中动物处置符合复旦大学实验动物伦理委员会要求。主要观察指标:术后1周行超声诊断仪检查室间隔缺损位置、最大分流速度,跨室间隔缺损处压差,肺动脉压力。结果:成活的3只草狗成功建立了室间隔缺损模型并进入结果分析。采用超声诊断仪于术后1周测得2只为嵴内型室间隔缺损,1只为膜部室间隔缺损;室间隔缺损直径1.8～3.6mm。测得室间隔缺损处最大分流速度为1.8～4.0m/s;跨室间隔缺损处压差42～51mmHg;肺动脉收缩压23～29mmHg。结论:在超声心动图定位下采用改良的主动脉-大隐静脉近端打孔器对室间隔穿刺可成功建立嵴内型/膜部室间隔缺损。BACKGROUND： Surrounding structure of intracristal ventricular septal defects （IVSD） and membranous ventricular septal defects （MVSD） is complicated, and it is difficult to perform transcatheter closure treatment. Corresponding animal models should be established to define an optimal interventional therapy. OBJECTIVE： To prepare the animal models of IVSD and MVSD under echocardiogram location. DESIGN： Animal modeling experiment. SETTING： Department of Cardiology, Zhongshan Hospital Affiliated to Fudan University. MATERIALS： Six healthy adult dogs, irrespective of gender, were purchased from Shanghai Experimental Animal Center. Aorta and great saphenous vein stapler-puncher （St.Jude corporation, USA） was used. The main improvement is to cut the plastic shell even, thin, or shorter in order to reduce the resistance when puncturing the ＂purse＂ in the right ventricle and the interventricular septum. Sonos 5 500 multifunction ultrasonoscope （Philips, USA） was used, and the frequency of the probe was between 2.5 3.5 MHz. METHODS： Experiments were performed at the Zhongshan Hospital Affiliated to Fudan University and Central Laboratory of Shanghai Institute of Cardiovascular Disease from April to July 2006 （biosafety level 2）. After anaesthesia, 6 adult dogs were fixed at decumbent position. The interventricular septum was punctured to make the preparation with the location of echocardiography （ECHO） by using the modified aorta and great saphenous vein proximal stapler-puncher. Animal intervention met the Animal Ethical Committee of Fudan University. MAIN OUTCOME MEASURES： One week after the surgery, transthoracic echocardiography examination was used to check the VSD site, the highest shunt flow rate, the pressure difference between both sides of the VSD, as well as pulmonary arterial pressure. RESULTS： The VSD models were successfully established in three survivals and included in the final result. Two models of IVSD and one model of MVSD were examined by ECHO one week

关 键 词：主动脉大隐静脉近端吻合器-打孔器 室间隔缺损 动物模型 医学工程 

分 类 号：R318[医药卫生—生物医学工程]