白星花金龟(Protaetia brevitarsis)幼虫抗菌物质的分离纯化  

Isolation and Purification of Antibacterial Materials from Protaetia brevitarsis (Coleoptera) Larva

作　　者：徐明旭[1] 高国富[1] 杨寿运[1] 孙捷[1] 张崇星[1] 徐春花[1] 吕毅 张克云[1] 

机构地区：[1]南京农业大学生命科学学院动物生物学系,中国江苏南京210095

出　　处：《生命科学研究》2008年第1期53-56,共4页Life Science Research

基　　金：江苏省自然科学基金资助项目(BK2006560);中国博士后科学基金资助项目(20060390292);江苏省博士后科学基金资助项目(0601030B)

摘　　要：以药用昆虫白星花金龟(Protaetia brevitarsis)幼虫为研究对象,通过Sephadex G-50、RP-HPLC等技术分离纯化天然抗菌活性物质.经初步鉴定,该系列抗菌活性物质对枯草芽孢杆菌(Bacillus subttilis)表现出较强的抑菌活性.用MALDI-TOFMS对样品进行分析,检测到相对分子质量分别为2254.0459、2622.4438的两种抗菌活性物质.推测这些抗菌活性物质在白星花金龟幼虫抵御微生物的侵袭中起到重要作用.Two antibacterial materials were purified from Protaetia brevitarsis larvae by Sephadex G-50 gel filtration and two-step reverse phase high performance liquid chromatography. The molecular weights were 2 254.045 9 and 2 262.443 8 Da respectively, determined by matrix-assisted laser desorption ionization timeof-flight mass spectrometry （MALDI-TOF）. They inhibited the growth of Bacillus subttilis ,which indicated that the two antibacterial materials should play an important role in preventing bacterial infection in P. brevitarsis.

关 键 词：白星花金龟 分离纯化 抗菌活性 

分 类 号：Q959[生物学—动物学]