杜仲药材高效液相色谱指纹图谱研究  

Fingerprint of Eucommia ulmoides Oliv by HPLC

作　　者：黄平[1] 席先蓉[1] 张恩景[2] 张琼[2] 韩双[1] 

机构地区：[1]贵阳中医学院,贵阳550002 [2]湖北中医学院,武汉430065

出　　处：《医药导报》2008年第4期376-378,共3页Herald of Medicine

摘　　要：目的应用高效液相色谱(HPLC)法对不同产地杜仲药材进行指纹图谱研究。方法XDB-C18色谱柱(150 mm×4.6 mm,5μm),乙腈-1%醋酸溶液二元梯度洗脱,体积流量1 mL.min-1,检测波长277 nm。结果根据选择的色谱条件制订了不同产地杜仲药材的指纹图谱。结论该方法操作简便,稳定,重复性好,可为更好地控制杜仲药材的内在质量提供科学依据。Objective To study a fingerprint of raw material of Eucommia ulmoides Oliv from various habitats by HPLC. Methods XDB-C18 column （ 150 mm×4.6 mm,5μm）was used,with mixtures of acetonitrile and 1% acetic acid as mobile phase in a gradient mode. The flow rate was 1 mL·min^-1 and wavelength was 277 nm. Results According to the optimum chromatographic conditions, a good fingerprint of Eucommia ulmoides Oliv were described out. Conclusion The method is simple and accurate with good reproducibility, which provides a scientific basis for controlling the quality of Eucommia ulmoides Oliv.

关 键 词：杜仲 色谱法 高效液相 指纹图谱 

分 类 号：R282.1[医药卫生—中药学]