复方抗病毒制剂体外抗流感病毒和呼吸道合胞病毒作用及毒性研究  

Toxibility and Effect of the Antiviral Herb Compound on Influenza Virus and Respiratory Syncytial Virus in Vitro

作　　者：杨道锋[1] 徐莉[1] 邵静芳[2] 张悦[2] 龚非力[2] 

机构地区：[1]华中科技大学同济医学院附属同济医院感染科,武汉430030 [2]华中科技大学同济医学院免疫学研究所,武汉430030

出　　处：《医药导报》2008年第4期392-394,共3页Herald of Medicine

摘　　要：目的测定复方抗病毒制剂体外对流感病毒和呼吸道合胞病毒的抗病毒作用。方法用鸡红细胞血凝素滴定法测定不同浓度复方抗病毒制剂在狗肾传代细胞(MDCK)中对流感病毒的抑制作用;用中和实验测定复方抗病毒制剂在Hep-2细胞中对呼吸道合胞病毒的活性。结果复方抗病毒制剂浓度<1.5 mg.mL-1时对MDCK细胞及Hep-2细胞无明显毒性。浓度>0.15 mg.mL-1能抑制流感病毒的血凝活性和呼吸道合胞病毒对靶细胞的攻击作用。结论复方抗病毒制剂体外对流感病毒和呼吸道合胞病毒具有较强的抑制作用,且有较大的安全性。Objective To investigate the antivirus activity of the antiviral herb compound on influenza virus and respiratory syncytial virus（RSV）. Methods The suppression on influenza virus by different doses of antiviral herb compound in MDCK passage cells was evaluated by hemaglutinin titration test, while the effect on RSV was studied by the neutralization test in Hep-2 cells. Results No obvious toxic effects on MDCK cells and Hep-2 cells were observed by the compound with the concentration lower than 1.5 mg·mL^-1. However, it suppressed the hemagglutination of influenza virus and the aggression of RSV on target cells at the concentration over 0. 15 mg·mL^-1. Conclusion The antiviral herb compound shows potent inhibition on the influenza virus and RSV in vitro with a high safety.

关 键 词：抗病毒制剂 复方 病毒 流感 合胞病毒 呼吸道 体外活性 

分 类 号：R285[医药卫生—中药学]