双波长薄层扫描法测定齐墩果酸片的含量  

Determination of Oleanolic Acid Tablets by Thin Layer Scanning

作　　者：杨辉[1] 赵咏[1] 李军[2] 

机构地区：[1]辽宁医学院药学院,锦州121001 [2]东北煤田地质局一五五勘探队

出　　处：《数理医药学杂志》2008年第2期207-208,共2页Journal of Mathematical Medicine

摘　　要：目的：建立齐墩果酸片的含量测定方法。方法：采用双波长薄层扫描法，以环己烷-丙酮-醋酸乙酯-冰醋酸（65：25：10：1）为展开剂，扫描波长,λS=220nm，λR=330nm。结果：齐墩果酸在1-5μg范围内线性关系良好（r=0．999），平均回收率99．11％，RSD为1．56％。结论：该法简便快速，结果稳定，重现性好。Objective：To establish a determination method of Oleanolic Acid Tablets. Methods：Thin layer scanning was used with cydohexane-acetone-ethyl acetate-acetic acid （65 ： 25 · 10： 1） as developer, 220nm as detection wavelength and 330nm as reference wavelength. Results： The calibration curve was linear in the range of 1-5μg （r= 0. 999）. The average recovery was 99. 11%, RSD was 1.56 %. Conclusion. This method was simple,rapid and the result was stable with a good repetition.

关 键 词：薄层扫描法 齐墩果酸 

分 类 号：R927.2[医药卫生—药学]