Te(Ⅳ)胁迫对两种螺旋藻生长及抗氧化酶系统的影响  

EFFECTS OF TELLURIUM STRESS ON THE GROWTH AND ANTIOXIDASE SYSTEM OF SPIRULINA PLATENSIS AND S.MAXIMUM

作　　者：陈小敏[1] 杨芳[1] 白燕[1] 周艳晖[1] 郑文杰[1] 

机构地区：[1]暨南大学化学系,广州510632

出　　处：《水生生物学报》2008年第2期148-153,共6页Acta Hydrobiologica Sinica

基　　金：广东自然科学基金重点项目(05103295);教育局重点项目(01141);广州市科技项目(2001-J-010-01)资助

摘　　要：在钝顶螺旋藻(Spirulina platensis)和极大螺旋藻(Spirulina maximum)接种的第5天至第10天添加不同浓度的亚碲酸钠,研究Te(Ⅳ)胁迫对两种螺旋藻的生长、抗氧化活性系统和脂质过氧化作用的影响。结果表明,通过调节添加碲的时间,可以有效地调节碲胁迫强度,随着加碲时间依次后移,碲胁迫强度逐渐减小,螺旋藻的最终生物量递增。碲对螺旋藻的生物效应与碲胁迫强度有关,也与添加碲时藻所处的生长期有关。实验组Ⅲ(分别在第7、8、第9天三天添加碲)的碲胁迫强度并不是最低的,两种螺旋藻的最终生物量也不是最大,但MDA的含量却是最低的,表明该实验组的生长状态是最佳的。各种抗氧化酶(SOD、GPX、CAT、APX和POD)的活性变化情况较复杂。其中,在碲胁迫下,GPX的活性显著提高。Spirulina, a blue-green algae, is gaining increasing attention because of its nutritional and medicinal properties, which have close relationship with anti-oxidation and free radical mechanism. In organism, the antioxidase system can effectively scavenge free radicals like O2^- . Recent research indicates that organo tellurium has immunomodulating property, antitumor and anticancer effects, which are similar to the functions of selenium, an essential trace mineral. We have reported that inorganic tellurium could be assimilated by both yeast and Spirulina. Moreover, our research also showed that adding different concentrations of tellurium to Spirulina had double effects on Spirulina, that is, tellurium would accelate the S. platensis growth when tellurium concentration was less than 2mg/L, but inhibit the S. platensis growth when tellurium concentration was bigger than 2mg/L, and S. platensis would stop growing or die when tellurium concentration was 320mg/L. Therefore, we can adjust that the stress intensity of tellurium on the growth of Spirulina by controlling the concentration of tellurium and the adding time. The objective of this study was to investigate the tellurium stress on the growth of two kinds of Spirulina, S. platensis and S. maximum, and to thor- oughly characterize the changes of antioxidase system in Spirulina, which would be helpful both to the research of biological function of tellurium and the biotransformation of tellurium by Spirulina. Different concentrations of tellurite were added to S. platensis and S. maximum on the 5^th to 10^th. The effects of Te （ Ⅳ ） stress on the growth, antioxidase system and lipid peroxidation level of S. platensis and S. maximum were investigated. It was indicated that the intensity of Te （ Ⅳ ） stress could be adjusted effectively by changing the time of adding Te （ Ⅳ ）. As the time of adding Te （ Ⅳ ） moved backwards, the intensity of Te （ Ⅳ ） stress decreased and the final biomass increased gradually. The biological effects of Te

关 键 词：碲 螺旋藻 抗氧化酶 碲胁迫强度 

分 类 号：Q178.54[生物学—水生生物学]