用流动注射法研究巯基化合物-铈(Ⅳ)-氢化可的松的化学发光反应  

Study on Chemiluminescence Reaction of Mercapto Compound-Cerium (Ⅳ)-Hydrocortisone by Flow Injection Analysis

作　　者：李怀娜[1] 慈云祥[2] 

机构地区：[1]曲阜师范大学化学系,曲阜273165 [2]北京大学化学与分子工程学院,北京100871

出　　处：《分析化学》1997年第6期679-682,共4页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金资助项目

摘　　要：采用流动注射法研究了巯基化合物－铈（Ⅳ）－氢化可的松（ＨＣＲｓ）体系的化学发光行 为，对影响化学发光强度的诸因素进行试验和探讨，建立了流动注射化学发光法检测谷胱甘 肽（ＧＳＨ），半胱氨酸（Ｃｙｓ）等含巯基化合物的新方法。检测ＧＳＨ和Ｃｙｓ和线性范围分别为 ２．０×１０－６～１０．０×１０－５ｍｏｌ／Ｌ和１．０×１０－６ｍｏｌ／Ｌ～１０．０×１０－５ｍｏｌ／Ｌ；检测限分别为２．０ ×１０－７ｍｏｌ／Ｌ和１．４×１０－６ｍｏｌ／Ｌ（Ｓ／Ｎ＝３）；ＧＳＨ、Ｃｙｓ加入血清中进行回收测定的回收率 为９０％～９５％，相对标准偏差小于４％。A new chemiluminescence (CL) reaction of -SH-Ce (Ⅳ )-HCRs was studied by flow-injection method in 1. 0 mol/L H2SO4. The factors influencing CL intensity were investigated. The new method based on CL reaction for the determination of glutathione (GSH) and cysteine (Cys ) has been established by optimizing the experimental conditions. The method allows the determination in the ranges of 2. 0 × 10-6 - 10. 0 × 10-5 mol/L for GSH and 1. 0 × 10-6- 10. 0 × 10-5 mol/L for Cys with detection limits of 2. 0 × 10-7 mol/L and 1. 4 × 10-6 mol/L (S/N= 3) for GSH and Cys respectively. The recoverics of GSH and Cys are between 90% and 95% from human serum.

关 键 词：化学发光 巯基化合物 铈 氢化可的松 GSH CYS 

分 类 号：Q517.03[生物学—生物化学] R446.112[医药卫生—诊断学]