薄层色谱-荧光熄灭法测定苦参碱的含量  

Determination of Matrine by Thin Layer Chromatography-Fluorescence Quenching

作　　者：汪宝琪[1] 庞志功[1] 王翔 胡晓璐[1] 

机构地区：[1]西安医科大学药学院,西安710061

出　　处：《分析化学》1997年第6期693-695,共3页Chinese Journal of Analytical Chemistry

摘　　要：用丙二酸和醋酐缩合自制成荧光试剂乙酸（β－二羧基α－甲基）乙烯酯，利用苦参碱对其有强烈的熄灭作用，达到定量测定苦参碱的目的。其线性范围为２×１０－６－２×１０－９ｇ／ｍＬ。最低检测限为１．４×１０－１１ｇ／ｍＬ；测得苦参碱和广豆根中苦参碱的含量分别为０．１６％和０．１１％。Using the property that matrine can strongly quench the fluorescnece of the reagent condensed from malonic acid and acetic anhydride, we determine the content of matrine. The linear range is 2× 10-6-2 × 10-9 g/mL and the lowest determination limit is 1. 4 × 10-11 g/mL. The content of matrine in Sophora Flarescens Ait and Sophora Tonkinensis Gapnep obtained by the present method is 0. 16% and 0. 11 %, respectively.

关 键 词：苦参碱 荧光 薄层色谱 苦参 山豆根 

分 类 号：R284.1[医药卫生—中药学] R979.1[医药卫生—中医学]