火麻仁蛋白对小鼠抗疲劳和免疫调节功能的初步研究  

Initial study of Hemp seeds protein on antifatigue and the immunomodulation effects in mice

作　　者：李永进[1] 杨睿悦[1] 扈学俸[1] 龙珠[1] 沈小毅[1] 李勇[1] 

机构地区：[1]北京大学医学部营养与食品卫生学系,北京100083

出　　处：《卫生研究》2008年第2期175-178,共4页Journal of Hygiene Research

基　　金：科技部国家重大支撑计划(No.2006BAD27B01;No.2006BAD27B08)

摘　　要：目的研究火麻仁蛋白对小鼠抗疲劳和免疫调节的作用。方法通过游泳时间、血乳酸和血清尿素氮测定观察小鼠的抗疲劳能力,应用免疫器官相对重量称重、细胞和体液免疫功能测定、以及脾脏T淋巴细胞亚群分类实验研究小鼠的相关免疫学指标。结果火麻仁蛋白能明显延长小鼠游泳时间、降低血乳酸值、增加肝糖原含量;并且火麻仁蛋白明显增强小鼠ConA诱导的脾淋巴细胞转化和迟发型变态反应,提高小鼠抗体生成数和半数溶血值,增强小鼠巨嗜细胞吞噬能力,增加小鼠外周血液中T淋巴细胞百分比。结论火麻仁蛋白可能增强小鼠抗疲劳能力和可增强小鼠免疫调节作用。Objective To study the effects of Hemp seeds protein（HSP） on antifatigue and immunnomodulation effect in mice. Methods Female ICR mice at the ages of 6 - 8 weeks were administered with the HSP for 4 weeks at the dose of 1.32,2.64 and 7.92 g/kg bw. After four weeks＇treatment, the mice swimming time and some biochemistry indexes were tested, such as blood lactic acid, BUN and hepatic glycogen, as well as the ratio of spleen and thymus organs index, tlae capacities of lymphocyte proliferation induced by ConA, DTH response, IgM - PFC number, the level of serum HC50, the indexes of the chicken red blood cells phagocytosis and the clearance rate of carbon particles were detected, and the ratio of CD4＋ T helper （Th） cell in spleen were determined by the flow cytometer. Results In comparison with the control group, the HSP could improve the the swimming time and contents of liver hepatin distinctly, and reduced the contents of blood lactic acid. Then the capacities of lymphocyte proliferation induced by ConA, DTH response, IgM - PFC number, the level of serum HC50 ,the indexes of the clearance rate of carbon particles and the ratio of CD/T helper （Th） cell in spleen increased. Conclusion HSP could have the ability of antifatigue and improve the immunomodulation effect＇in mice.

关 键 词：火麻仁蛋白 抗疲劳 免疫力 

分 类 号：Q51[生物学—生物化学] R961[医药卫生—药理学]