我国海南省杀蚊球形芽孢杆菌的分离和分布  

ISOLATION OF MOSQUITO-LARVICIDAL BACILLUS SPHAERICUS FROM THE SOIL SAMPLES COLLECTED IN HAINAN, CHINA

作　　者：袁志明[1] 陈宗胜[1] 刘娥英[1] 蔡全信[1] 张用梅[1] 

机构地区：[1]中国科学院武汉病毒研究所,武汉43007

出　　处：《微生物学通报》1997年第4期203-205,共3页Microbiology China

基　　金：国家自然科学基金;中国科学院微生物所做生物资源前期开发实验室资助

摘　　要：在我国海南省共采集158个各种类型的泥土样品，采用选择性培养基（PBMYS）进行样品分离，并结合形态学观察、血清学鉴定和生物测定，获得对致倦库蚊（Culexquinquefasciatus）幼虫有毒性的球形芽孢杆菌（Bacillussphaericus）73株，有毒株的分离率占样品总数的342％。血清学试验表明，新分离菌株有71株属H5a5b型，两株属在我国首次分离出比型菌株。许多球形芽bqn杆菌新菌株对致倦库蚊幼虫具有很强的毒杀作用。其中228－2和117－1菌株的丙酮粉剂效价分别为2721ITU／mg和2331ITU／mg，高于相同条件下制备的参考菌株2362丙团粉剂的效价（2218ITU／mg）。During 1992-1993, one hundred and fifty eight of vanous soil samples collected in fourteen counties or cities in Hainan Province. With a selected medium (PBMYS), totally seventy-three strains of Bacillus sphaericus, possessing larvicidal achvity against Culex quinquefasciatus, were isolated. The rate of active strains isolated is 34.2% in the total of samples. The seroIogical study showed that most of newly isolates were belonging to serotype Wash except that tWo strains, 47-6b and 124-3, were belonging to H6.The acetone powders prepared from several newly isolates of H5aa5b, were highiy toxic to the larvae of C quinquefasciatus. Two of them, 228-2 and 117-1, had a calculated Potency of 2721 and 2331ITU/ mg respechvely. In contraSt with the potent sthen 2362, it had a potency value of 2218ITU/mg.

关 键 词：昆虫细菌 球形芽孢杆菌 灭蚊 分离 分布 

分 类 号：S476.11[农业科学—农业昆虫与害虫防治] R184.31[农业科学—植物保护]