三羟基异黄酮抑制增生性瘢痕成纤维细胞转分化作用的研究  

Genistein inhibits transdifferentiation of human hypertrophic scar fibroblasts in vitro

作　　者：曹川[1] 李世荣[1] 覃霞[1] 陈艳清[1] 姚恒[1] 冯智[1] 李喆[1] 

机构地区：[1]第三军医大学西南医院整形外科,重庆400038

出　　处：《第三军医大学学报》2008年第7期618-620,共3页Journal of Third Military Medical University

摘　　要：目的观察三羟基异黄酮(Genistein)对人增生性瘢痕(hypertrophic scar,HS)成纤维细胞向肌成纤维细胞转分化的影响,探讨其抑制组织纤维化的作用机制。方法体外培养人HS成纤维细胞,分别以25、50、100μmol/L浓度的Genistein进行处理,流式细胞术检测体外培养HS成纤维细胞中α-SMA阳性细胞率;Western blot检测细胞α-SMA蛋白的表达;观察HS成纤维细胞三维培养组织的收缩情况。结果在Genistein作用下,α-SMA阳性细胞率及α-SMA蛋白表达与对照组比较均降低(P<0.05);其中50、100μmol/L组与对照相比较具有非常显著性差异(P<0.01)。HS成纤维细胞三维培养组织持续收缩,加入Genistein作用后,组织块的收缩现象减弱,第48、72小时,50μmol/L与100μmol/L组收缩指数(CI)显著低于对照组(P<0.01),呈现明显的时效-剂量关系。结论Genistien能抑制HS成纤维细胞向肌成纤维细胞的转分化,可能是其抗组织纤维化的作用机制之一。Objective To observe the effects of genistein on the transdifferentiation of the cultured human hypertrophic scar fibroblasts and to explore the mechanism of the anti-fibrosis by genistein. Methods Human hypertrophic scar fibroblasts were cultured in presence of genistein （25, 50, and 100 μmol/L respectively）. The rate of α-SMA-positive cells was measured with flow cytometry and the expression of α-SMA protein was detected by Western blotting. The fibroblasts in 3D gel culture were established and their contraction was measured 24, 48, and 72 h after genistein treatment. Results After the treatment with genistein, the rate of α- SMA-positive cells and the expression of α-SMA protein were significantly decreased （P 〈 0.05 ） , especially when treated with 50 and 100 μmol/L genistein （P 〈0.01 ）. In fibroblasts in 3D gel culture, the control cells kept contracting. The contraction of the experimental cells was weakened after genistein treatment in a dose-dependent and time-dependent manner. Conclusion Genistein can inhibit the transdifferentiation of the cultured human hypertrophic scar fibroblasts to myofibroblasts, which may be one of the important anti-fibrosis mechanisms.

关 键 词：转分化 三羟基异黄酮 增生性瘢痕 成纤维细胞 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学] R364.31[医药卫生—基础医学]