检索规则说明:AND代表“并且”;OR代表“或者”;NOT代表“不包含”;(注意必须大写,运算符两边需空一格)
检 索 范 例 :范例一: (K=图书馆学 OR K=情报学) AND A=范并思 范例二:J=计算机应用与软件 AND (U=C++ OR U=Basic) NOT M=Visual
机构地区:[1]第三军医大学基础医学部全军免疫学研究所,重庆400038 [2]解放军信息工程大学卫生处,郑州450000
出 处:《第三军医大学学报》2008年第7期625-627,共3页Journal of Third Military Medical University
摘 要:目的观察esp1(estradiol-stimulated protein)在A549和Hela两种肿瘤细胞株中的表达与染色体倍性的关系。方法提取肿瘤细胞A549、Hela及分别转染pIRE-S2-EGFP-ESP1的相应肿瘤细胞总RNA,逆转录,real-time quantitative PCR检测,比较esp1在肿瘤细胞中的表达变化;VIDEOTEST-KARYO3.1分析相应的染色体数目变化;流式细胞仪检测其染色体倍性的改变。结果转染pIRE-S2-EGFP-ESP1后esp1表达上调(P<0.01),染色体数目减少(P<0.01),染色体倍性降低(P<0.01)。结论esp1上调表达可以抑制肿瘤细胞异常分裂相,降低染色体倍性,但是esp1在A549和Hela两种肿瘤细胞株中的表达没有观察到明显差异。Objective To observe the relation of estradiol-stimulated protein (esp1) expression with chromosome ploidy in A549 and Hela cells. Methods Total RNA was extracted from A549 and Hela cells respectively for cDNA. pIRE-S2-EGFP-ESP1 plasmid was transfected into the 2 corresponding tumor cells. Real-time quantitative PCR was used to detect esp1 gene expression. The chromosome changes were analyzed by VIDEOTEST-KARYO 3.1 and chromosome ploidy was analyzed with flow cytometry. Results After the tumor cells were transfected with pIRE-S2-EGFP-ESP1 plasmid, the expression of esp1 gene was up-regulated (P 〈 0.01 ), chromosome number decreased ( P 〈 0.01 ), and chromosome ploidy reduced (P 〈 0.01 ). Conclusion The over-expression of esp1 gene inhibits the abnormal proliferation of tumor cells and decreases chromosome ploidy.
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在链接到云南高校图书馆文献保障联盟下载...
云南高校图书馆联盟文献共享服务平台 版权所有©
您的IP:216.73.216.124