新鲜羊膜与不同保存方式羊膜眼表移植后转归的实验研究  

Outcomes of different-method-preserved amniotic membrane graft after transplanted to rabbit occular surface

作　　者：赵敏[1] 胡柯[1] 张琪[1] 李鸿[1] 

机构地区：[1]重庆医科大学附属第一医院眼科,重庆400016

出　　处：《第三军医大学学报》2008年第7期634-636,共3页Journal of Third Military Medical University

基　　金：重庆市科委科学基金[渝科委计(2003-23)]~~

摘　　要：目的观察比较新鲜羊膜(fresh amniotic membrane,FAM)、甘油保存羊膜(glycerol-preserved amniotic membrane,GPAM)、100%乙醇保存羊膜(alcohol-preserved amniotic membrane,APAM)、真空干燥羊膜(vacuum preserved amniotic membrane,VXAM)移植于碱烧伤的兔眼表后在眼表的变化及其最终结局。方法建立兔角膜碱烧伤模型,将36只新西兰大白兔随机分为FAM、GPAM、APAM、VXAM4组,每组9只,术后裂隙灯显微镜观察眼表治疗效果及羊膜植片变化情况;术后10、14、30d作组织病理HE染色。结果FAM组治疗效果最好,APAM组治疗效果不明显。移植后植片10d左右开始溶解,至14d左右全部溶解脱落。APAM组羊膜溶解时间较晚,FAM组溶解最快。溶解后在各时间点各组组织切片标本中均未找到羊膜组织。GPAM及VXAM间治疗效果相似。结论羊膜移植后最终溶解脱落,不能与受体组织融合,角膜上皮在其下生长。羊膜眼表移植严格意义上只能叫做羊膜敷贴术。Objective To compare the effects of fresh amniotic membrane （FAM）, glycerol-preserved amniotic membrane （GPAM）, alcohol-preserved amniotic membrane （APAM） and amniotic membrane preserved under vacuum drying condition （VXAM） on rabbit corneal alkali burn and to observe the outcomes of the grafted amniotic membrane. Methods Thirty-six New Zealand rabbits were used to establish corneal alkali burn models and then divided into 4 groups according different preserved amniotic membrane they received （FAM, GPAM, VXAM and APAM）, with 9 rabbits in each group. After transplantation, we observed the changes of amniotic membrane graft, corneal opacity, corneal neovascularization （CNV） with slit lamp microscopy. The amniotic membrane graft was examined by HE staining 10 d, 14 d and 1 month after transplantation. Results One month later, FAM graft, GPAM graft and VXAM graft showed better curative effect than APAM graft. Ten days after transplantation, the amniotic membrane graft began to dissolve and desquamate, and disappeared completely till 14 d after transplantation. Amniotic membrane tissues were not found under light microscope in any rabbit. FAM graft dissolved most quickly, and APAM graft was the most slowly. The dissolution of GPAM graft and VXAM graft had no significant difference. Conclusion After amniotic membrane transplantation, it dissolves and sheds instead of fusing with recipient tissues. Corneal epithelium grows under amniotic membrane graft. Amniotic membrane graft transplantation should be called as amniotic membrane patching.

关 键 词：羊膜 碱烧伤 转归 

分 类 号：R318.08[医药卫生—生物医学工程] R779.6[医药卫生—基础医学]